Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Plexin-B2 (PLXNB2) Standard Curve.)
Human Plexin-B2 (PLXNB2) ELISA Kit | PLXNB2 elisa kit
Human Plexin-B2 (PLXNB2) ELISA Kit
Gene Names
PLXNB2; MM1; PLEXB2; Nbla00445; dJ402G11.3
Reactivity
Human
Synonyms
Plexin-B2 (PLXNB2); Human Plexin-B2 (PLXNB2) ELISA Kit; PLXNB2; RP3-402G11.19; KIAA0315; MM1; Nbla00445; PLEXB2; dJ402G11.3; OTTHUMP00000196636; PLXNB2 elisa kit
Reactivity
Human
Specificity
Human Plexin-B2 (PLXNB2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PLXNB2. No significant cross-reactivity or interference between Human PLXNB2 and analogues was observed.
Sequence Length
522
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Plexin-B2 (PLXNB2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Plexin-B2 (PLXNB2) Standard Curve.)
Related Product Information for PLXNB2 elisa kit
Description: This Human Plexin-B2 (PLXNB2) ELISA Kit employs a two-site sandwich ELISA to quantitate PLXNB2 in samples. An antibody specific for PLXNB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLXNB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLXNB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLXNB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Members of the B class of plexins, such as PLXNB2 is related to human SEP (PLXNB1). RT-PCR detected highest PLXNB2 expression in testis and ovary, followed by placenta, lung, liver, and kidney. Much lower expression was detected in prostate and small intestine, and little to none was detected in other tissues examined. Ligand-induced dimerization of plexin B was sufficient to stimulate endogenous RhoA (ARHA) potently and induce the reorganization of the cytoskeleton in mammalian cells. Overexpression of the PDZ domain of PDZ-RhoGEF prevented cell rounding and neurite retraction in differentiated rat neural precursor cells induced by activation of endogenous PLXNB1 by semaphorin-4D.
Background: Members of the B class of plexins, such as PLXNB2 is related to human SEP (PLXNB1). RT-PCR detected highest PLXNB2 expression in testis and ovary, followed by placenta, lung, liver, and kidney. Much lower expression was detected in prostate and small intestine, and little to none was detected in other tissues examined. Ligand-induced dimerization of plexin B was sufficient to stimulate endogenous RhoA (ARHA) potently and induce the reorganization of the cytoskeleton in mammalian cells. Overexpression of the PDZ domain of PDZ-RhoGEF prevented cell rounding and neurite retraction in differentiated rat neural precursor cells induced by activation of endogenous PLXNB1 by semaphorin-4D.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
PLXNB2 protein
NCBI Official Synonym Full Names
plexin B2
NCBI Official Symbol
PLXNB2
NCBI Official Synonym Symbols
MM1; PLEXB2; Nbla00445; dJ402G11.3
NCBI Protein Information
plexin-B2
UniProt Protein Name
Plexin-B2
Protein Family
UniProt Gene Name
PLXNB2
UniProt Synonym Gene Names
KIAA0315
NCBI Description
Members of the B class of plexins, such as PLXNB2 are transmembrane receptors that participate in axon guidance and cell migration in response to semaphorins (Perrot et al. (2002) [PubMed 12183458]).[supplied by OMIM, Mar 2008]
Uniprot Description
Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling. Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal differentiation and migration of neuronal cells during brain corticogenesis and for normal embryonic brain development. Regulates the migration of cerebellar granule cells in the developing brain. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. May modulate the activity of RAC1 and CDC42. Down-regulates macrophage migration in wound-healing assays (in vitro) ().
Research Articles on PLXNB2
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Product Notes
The Human PLXNB2 plxnb2 (Catalog #AAA9714867) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714867 ELISA Kit recognizes Human PLXNB2. It is sometimes possible for the material contained within the vial of "Plexin-B2 (PLXNB2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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