Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Platelet-activating factor receptor (PTAFR) Standard Curve.)
Human Platelet-Activating Factor Receptor (PTAFR) ELISA Kit | PTAFR elisa kit
Human Platelet-Activating Factor Receptor (PTAFR) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Platelet-activating factor receptor (PTAFR) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Platelet-activating factor receptor (PTAFR) Standard Curve.)
Background: The platelet-activating factor receptor is a G-protein coupled receptor which binds platelet-activating factor.The PAF receptor shows structural characteristics of the rhodopsin gene family and binds platelet-activating factor (PAF). PAF is a phospholipid (1-0-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine) that has been implicated as a mediator in diverse pathologic processes, such as allergy, asthma, septic shock, arterial thrombosis, and inflammatory processes. PTAFR has 2 N-glycosylation sites, but unlike other rhodopsin family members, there is no N-glycosylation site in the N-terminal segment. Transfection of PTAFR into COS-7 cells resulted in expression of the receptor with an extracellular N terminus.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a seven-transmembrane G-protein-coupled receptor for platelet-activating factor (PAF) that localizes to lipid rafts and/or caveolae in the cell membrane. PAF (1-0-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine) is a phospholipid that plays a significant role in oncogenic transformation, tumor growth, angiogenesis, metastasis, and pro-inflammatory processes. Binding of PAF to the PAF-receptor (PAFR) stimulates numerous signal transduction pathways including phospholipase C, D, A2, mitogen-activated protein kinases (MAPKs), and the phosphatidylinositol-calcium second messenger system. Following PAFR activation, cells become rapidly desensitized and this refractory state is dependent on PAFR phosphorylation, internalization, and down-regulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]
Uniprot Description
PTAFR: Receptor for platelet activating factor, a chemotactic phospholipid mediator that possesses potent inflammatory, smooth- muscle contractile and hypotensive activity. Seems to mediate its action via a G protein that activates a phosphatidylinositol- calcium second messenger system. Belongs to the G-protein coupled receptor 1 family.
Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, family 1
Chromosomal Location of Human Ortholog: 1p35-p34.3
Cellular Component: nucleoplasm; membrane; intracellular membrane-bound organelle; integral to plasma membrane; plasma membrane; integral to membrane
Molecular Function: G-protein coupled receptor activity; platelet activating factor receptor activity; purinergic nucleotide receptor activity, G-protein coupled; lipopolysaccharide binding; lipopolysaccharide receptor activity; phospholipid binding
Biological Process: G-protein coupled receptor protein signaling pathway; phosphoinositide-mediated signaling; inositol trisphosphate biosynthetic process; cytokine and chemokine mediated signaling pathway; lipopolysaccharide-mediated signaling pathway; cytokine production; immune response; chemotaxis; inflammatory response
Research Articles on PTAFR
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Product Notes
The Human PTAFR ptafr (Catalog #AAA9714742) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714742 ELISA Kit recognizes Human PTAFR. It is sometimes possible for the material contained within the vial of "Platelet-Activating Factor Receptor (PTAFR), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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