SDS-Page
AGRP recombinant protein
AGRP, 21-132aa, Human, His tag, E Coli
Gene Names
AGRP; ART; AGRT; ASIP2
Applications
SDS-Page
Purity
> 85% by SDS-PAGE
Synonyms
AGRP; 21-132aa; Human; His tag; E Coli; Agouti-related protein; AGRT; ART; ASIP2; AGRP recombinant protein
Host
E Coli
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl (pH 8.5) containing 20% glycerol, 1mM DTT.
Concentration
0.25mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAQMGL APMEGIRRPD QALLPELPGL GLRAPLKKTT AEQAEEDLLQ EAQALAEVLD LQDREPRSSR RCVRLHESCL GQQVPCCDPC ATCYCRFFNA FCYCRKLGTA MNPCSRT
Sequence Length
132
Applicable Applications for AGRP recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for AGRP recombinant protein
AGRP, also known as agouti-related protein also called agouti-related peptide, is a neuropeptide produced in the brain by the AGRP/NPY neuron. It is only synthesised in NPY-containing cell bodies located in the ventromedial part of the arcuate nucleus in the hypothalamus. AGRP is co-expressed with Neuropeptide Y and works by increasing appetite and decreasing metabolism and energy expenditure. It is one of the most potent and long-lasting of appetite stimulators. Recombinant human AGRP, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for AGRP recombinant protein
References
Xiao E, Xia-Zhang L, et al. (2003). Endocrinology. 144 (5):1736-41.; Creemers JW, Pritchard LE, et al. (2006). Endocrinology. 147 (4):1621-31.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15.1kDa (137aa) confirmed by MALDI-TOF.
NCBI Official Full Name
agouti-related protein
NCBI Official Synonym Full Names
agouti related neuropeptide
NCBI Official Symbol
AGRP
NCBI Official Synonym Symbols
ART; AGRT; ASIP2
NCBI Protein Information
agouti-related protein
UniProt Protein Name
Agouti-related protein
Protein Family
UniProt Gene Name
AGRP
UniProt Synonym Gene Names
AGRT; ART
NCBI Description
This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity. [provided by RefSeq, Dec 2009]
Uniprot Description
Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R (). Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner.
Research Articles on AGRP
Similar Products
Product Notes
The AGRP agrp (Catalog #AAA206129) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's AGRP can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the AGRP agrp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSHMAQMGL APMEGIRRPD QALLPELPGL GLRAPLKKTT AEQAEEDLLQ EAQALAEVLD LQDREPRSSR RCVRLHESCL GQQVPCCDPC ATCYCRFFNA FCYCRKLGTA MNPCSRT. It is sometimes possible for the material contained within the vial of "AGRP, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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