Western Blot (WB)
(Western Blot analysis in A549 whole cell lysate (20ug) using MBS647791 (1:1000). Secondary IgG (HRP) Gtx Rb at 1:10,000. Predicted band size: 39kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human, Mouse PTAFR Polyclonal Antibody | anti-PTAFR antibody
PTAFR, CT (PTAFR, PAFR, Platelet-activating factor receptor)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Western Blot (WB)
(Western Blot analysis in A549 whole cell lysate (20ug) using MBS647791 (1:1000). Secondary IgG (HRP) Gtx Rb at 1:10,000. Predicted band size: 39kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western Blot analysis in A549 whole cell lysate (20ug) using MBS647791 (1:1000). Secondary IgG (HRP) Gtx Rb at 1:10,000. Predicted band size: 39kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a seven-transmembrane G-protein-coupled receptor for platelet-activating factor (PAF) that localizes to lipid rafts and/or caveolae in the cell membrane. PAF (1-0-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine) is a phospholipid that plays a significant role in oncogenic transformation, tumor growth, angiogenesis, metastasis, and pro-inflammatory processes. Binding of PAF to the PAF-receptor (PAFR) stimulates numerous signal transduction pathways including phospholipase C, D, A2, mitogen-activated protein kinases (MAPKs), and the phosphatidylinositol-calcium second messenger system. Following PAFR activation, cells become rapidly desensitized and this refractory state is dependent on PAFR phosphorylation, internalization, and down-regulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]
Uniprot Description
Function: Receptor for platelet activating factor, a chemotactic phospholipid mediator that possesses potent inflammatory, smooth-muscle contractile and hypotensive activity. Seems to mediate its action via a G protein that activates a phosphatidylinositol-calcium second messenger system. Ref.1 Ref.2 Ref.3 Ref.5
Subunit structure: Interacts with ARRB1. Ref.13
Subcellular location: Cell membrane; Multi-pass membrane protein Ref.3.
Tissue specificity: Expressed in the placenta, lung, left and right heart ventricles, heart atrium, leukocytes and differentiated HL-60 granulocytes. Ref.1 Ref.2 Ref.5
Induction: By CSF2/GM-CSF, IL5/interleukin-5 and n-butyrate. Ref.2
Sequence similarities: Belongs to the G-protein coupled receptor 1 family.
Research Articles on PTAFR
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Product Notes
The PTAFR ptafr (Catalog #AAA647791) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PTAFR, CT (PTAFR, PAFR, Platelet-activating factor receptor) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PTAFR can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the PTAFR ptafr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTAFR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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