Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse DAZ-associated protein 2 (DAZAP2) Standard Curve.)
Mouse DAZ-Associated Protein 2 (DAZAP2) ELISA Kit | DAZAP2 elisa kit
Mouse DAZ-Associated Protein 2 (DAZAP2) ELISA Kit
Gene Names
Dazap2; Brbp; Prtb; Gcap28; gt6-12; AI314727; mKIAA0058
Reactivity
Mouse
Synonyms
DAZ-Associated Protein 2 (DAZAP2); Mouse DAZ-Associated Protein 2 (DAZAP2) ELISA Kit; DAZAP2; KIAA0058; MGC14319; MGC766; PRTB; deleted in azoospermia associated protein 2; proline-rich transcript in brain; proline-rich transcript; brain-expressed protein; DAZAP2 elisa kit
Reactivity
Mouse
Specificity
Mouse DAZ-associated protein 2 (DAZAP2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DAZAP2. No significant cross-reactivity or interference between Mouse DAZAP2 and analogues was observed.
Sequence Length
168
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse DAZ-associated protein 2 (DAZAP2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse DAZ-associated protein 2 (DAZAP2) Standard Curve.)
Related Product Information for DAZAP2 elisa kit
Description: This Mouse DAZ-associated protein 2 (DAZAP2) ELISA Kit employs a two-site sandwich ELISA to quantitate DAZAP2 in samples. An antibody specific for DAZAP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DAZAP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DAZAP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DAZAP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DAZ-associated protein 2 (DAZAP2) encodes a proline-rich protein which interacts with the deleted in azoospermia (DAZ) and the deleted in azoospermia-like gene through the DAZ-like repeats. This protein also interacts with the transforming growth factor-beta signaling molecule SARA (Smad anchor for receptor activation), eukaryotic initiation factor 4G, and an E3 ubiquitinase that regulates its stability in splicing factor containing nuclear speckles. The encoded protein may function in various biological and pathological processes including spermatogenesis, cell signaling and transcription regulation, formation of stress granules during translation arrest, RNA splicing, and pathogenesis of multiple myeloma. Multiple transcript variants encoding different isoforms have been found for this gene.
Background: DAZ-associated protein 2 (DAZAP2) encodes a proline-rich protein which interacts with the deleted in azoospermia (DAZ) and the deleted in azoospermia-like gene through the DAZ-like repeats. This protein also interacts with the transforming growth factor-beta signaling molecule SARA (Smad anchor for receptor activation), eukaryotic initiation factor 4G, and an E3 ubiquitinase that regulates its stability in splicing factor containing nuclear speckles. The encoded protein may function in various biological and pathological processes including spermatogenesis, cell signaling and transcription regulation, formation of stress granules during translation arrest, RNA splicing, and pathogenesis of multiple myeloma. Multiple transcript variants encoding different isoforms have been found for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
DAZ-associated protein 2
NCBI Official Synonym Full Names
DAZ associated protein 2
NCBI Official Symbol
Dazap2
NCBI Official Synonym Symbols
Brbp; Prtb; Gcap28; gt6-12; AI314727; mKIAA0058
NCBI Protein Information
DAZ-associated protein 2
UniProt Protein Name
DAZ-associated protein 2
Protein Family
UniProt Gene Name
Dazap2
UniProt Synonym Gene Names
Prtb
UniProt Entry Name
DAZP2_MOUSE
Research Articles on DAZAP2
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Product Notes
The Mouse DAZAP2 dazap2 (Catalog #AAA9717937) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717937 ELISA Kit recognizes Mouse DAZAP2. It is sometimes possible for the material contained within the vial of "DAZ-Associated Protein 2 (DAZAP2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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