Rabbit anti-Human SPG7 Polyclonal Antibody | anti-SPG7 antibody

SPG7, ID (SPG7, CAR, CMAR, PGN, Paraplegin, Spastic paraplegia 7 protein) (AP)

Cat. Num. AAA6350519

• Gene Names: SPG7; CAR; PGN; CMAR; SPG5C
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SPG7; Polyclonal Antibody; ID (SPG7; CAR; CMAR; PGN; Paraplegin; Spastic paraplegia 7 protein) (AP); Spastic paraplegia 7 protein; anti-SPG7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 489

• Applicable Applications for anti-SPG7 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SPG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 114-141 amino acids from the Central region of human SPG7.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SPG7 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the SPG7 antibody detected the SPG7 protein (arrow).)

Immunohistochemistry (IHC)

(SPG7 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPG7 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-SPG7 antibody

This gene encodes a nuclear-encoded mitochondrial metalloprotease protein that is a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Two transcript variants encoding distinct isoforms have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 7. [provided by RefSeq].

Product Categories/Family for anti-SPG7 antibody

Antibodies; Proteins

References

Warnecke, T., et al. Mov. Disord. 25(4):413-420(2010). Augustin, S., et al. Mol. Cell 35(5):574-585(2009). Karlberg, T., et al. PLoS ONE 4 (10), E6975 (2009): Brugman, F., et al. Neurology 71(19):1500-1505(2008). Tzoulis, C., et al. J. Neurol. 255(8):1142-1144(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 40806173
• NCBI GeneID: 6687
• NCBI Accession #: NP_955399.1; NP_003110.1
• NCBI GenBank Nucleotide #: NP_955399.1
• UniProt Accession #: Q9UQ90
• NCBI Official Full Name: paraplegin isoform 2
• NCBI Official Synonym Full Names: SPG7 matrix AAA peptidase subunit, paraplegin
• NCBI Official Symbol: SPG7
• NCBI Official Synonym Symbols: CAR; PGN; CMAR; SPG5C
• NCBI Protein Information: paraplegin
• UniProt Protein Name: Paraplegin
• Protein Family: Paraplegin
• UniProt Gene Name: SPG7
• UniProt Synonym Gene Names: CAR; CMAR; PGN
• UniProt Entry Name: SPG7_HUMAN

NCBI Description

This gene encodes a mitochondrial metalloprotease protein that is a member of the AAA family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Mutations in this gene cause autosomal recessive spastic paraplegia 7. Two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2014]