Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide (DAPP1) Standard Curve.)
Human Dual Adapter For Phosphotyrosine And 3-Phosphotyrosine And 3-Phosphoinositide (DAPP1) ELISA Kit | DAPP1 elisa kit
Human Dual Adapter For Phosphotyrosine And 3-Phosphotyrosine And 3-Phosphoinositide (DAPP1) ELISA Kit
Gene Names
DAPP1; BAM32
Reactivity
Human
Synonyms
Dual Adapter For Phosphotyrosine And 3-Phosphotyrosine And 3-Phosphoinositide (DAPP1); Human Dual Adapter For Phosphotyrosine And 3-Phosphotyrosine And 3-Phosphoinositide (DAPP1) ELISA Kit; DAPP1; BAM32; DKFZp667E0716; DAPP1 elisa kit
Reactivity
Human
Specificity
Human Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide (DAPP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DAPP1. No significant cross-reactivity or interference between Human DAPP1 and analogues was observed.
Sequence Length
280
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide (DAPP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide (DAPP1) Standard Curve.)
Related Product Information for DAPP1 elisa kit
Description: This Human Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide (DAPP1) ELISA Kit employs a two-site sandwich ELISA to quantitate DAPP1 in samples. An antibody specific for DAPP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DAPP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DAPP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DAPP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide is a protein that in humans is encoded by the DAPP1 gene.
Background: Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide is a protein that in humans is encoded by the DAPP1 gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide isoform 1
NCBI Official Synonym Full Names
dual adaptor of phosphotyrosine and 3-phosphoinositides 1
NCBI Official Symbol
DAPP1
NCBI Official Synonym Symbols
BAM32
NCBI Protein Information
dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide
UniProt Protein Name
Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide
UniProt Gene Name
DAPP1
UniProt Synonym Gene Names
BAM32; hDAPP1
UniProt Entry Name
DAPP1_HUMAN
Uniprot Description
DAPP1: an adaptor protein. Regulates antigen receptor signaling downstream of phosphatidylinositol 3-kinase.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 4q25-q27
Cellular Component: plasma membrane; cytosol
Molecular Function: phosphatidylinositol-3,4,5-triphosphate binding; phospholipid binding; phosphatidylinositol-3,4-bisphosphate binding
Biological Process: signal transduction; protein amino acid dephosphorylation
Research Articles on DAPP1
Similar Products
Product Notes
The Human DAPP1 dapp1 (Catalog #AAA9715808) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715808 ELISA Kit recognizes Human DAPP1. It is sometimes possible for the material contained within the vial of "Dual Adapter For Phosphotyrosine And 3-Phosphotyrosine And 3-Phosphoinositide (DAPP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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