Rabbit MITF Polyclonal Antibody | anti-MITF antibody

MITF Antibody (N-term)

Cat. Num. AAA9210845

• Gene Names: MITF; MI; WS2; CMM8; WS2A; bHLHe32
• Reactivity: Human (Predicted Reactivity: Mouse)
• Applications: Western Blot, ELISA, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: MITF; Polyclonal Antibody; MITF Antibody (N-term); Microphthalmia-associated transcription factor; Class E basic helix-loop-helix protein 32; bHLHe32; BHLHE32; anti-MITF antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This MITF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-28 amino acids from the N-terminal region of human MITF.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 1-28
• Sequence Length: 419

• Applicable Applications for anti-MITF antibody: Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-MITF Antibody (N-term)at 1:2000 dilution + A375 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 59 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(MITF Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MITF antibody detected the MITF protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of MITF Antibody (N-term) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(MITF Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MITF antibody

This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

Product Categories/Family for anti-MITF antibody

Immunology

References

Wang, Y., et al. BMC Med 8, 14 (2010) : Shiohara, M., et al. Int J Lab Hematol 31(2):215-226(2009) Hershey, C.L., et al. Gene 347(1):73-82(2005) Miller, A.J., et al. J. Biol. Chem. 280(1):146-155(2005) Shibahara, S., et al. J. Investig. Dermatol. Symp. Proc. 6(1):99-104(2001)

NCBI and Uniprot Product Information

• NCBI GI #: 4557755
• NCBI GeneID: 4286
• NCBI Accession #: NP_000239.1; NP_001171896.1; NP_006713.1; NP_937801.1; NP_937802.1; NP_937820.1; NP_937821.2
• NCBI GenBank Nucleotide #: NM_000248.3
• UniProt Accession #: O75030; Q14841; Q9P2V0; Q9P2V1; Q9P2V2; Q9P2Y8; B4DJL2; D3K197; E9PFN0
• Molecular Weight: 58795
• NCBI Official Full Name: microphthalmia-associated transcription factor isoform 4
• NCBI Official Synonym Full Names: microphthalmia-associated transcription factor
• NCBI Official Symbol: MITF
• NCBI Official Synonym Symbols: MI; WS2; CMM8; WS2A; bHLHe32
• NCBI Protein Information: microphthalmia-associated transcription factor
• UniProt Protein Name: Microphthalmia-associated transcription factor
• Protein Family: Microphthalmia-associated transcription factor
• UniProt Gene Name: MITF
• UniProt Synonym Gene Names: BHLHE32; bHLHe32
• UniProt Entry Name: MITF_HUMAN

NCBI Description

This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

MITF: a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. Plays a critical role in the differentiation of various cell types including neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. Two isoforms are known: the M-isoform is expressed exclusively in melanocytes, while the A-isoform has a much broader range of expression. Mutations in MITF can lead to Waardenburg syndrome. Ten alternatively spliced isoforms have been described.

Protein type: Oncoprotein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 3p14.2-p14.1

Cellular Component: protein complex; nucleus

Molecular Function: protein dimerization activity; RNA polymerase II transcription factor activity, enhancer binding; protein binding; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; camera-type eye development; regulation of osteoclast differentiation; cell fate commitment; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; melanocyte differentiation; protein complex assembly; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; osteoclast differentiation; bone remodeling; regulation of cell proliferation

Disease: Waardenburg Syndrome, Type 2a; Albinism, Ocular, With Sensorineural Deafness; Melanoma, Cutaneous Malignant, Susceptibility To, 8; Tietz Syndrome