Human Parvovirus B19 IgM ELISA Kit
Parvovirus B19 IgM ELISA
Reactivity
Human
Synonyms
Parvovirus B19 IgM; Parvovirus B19 IgM ELISA; Parvovirus B19 IgM elisa kit
Reactivity
Human
Specificity
The specificity is defined as the probability of the assay of scoring negative in the absence of the specific analyte. (Detected by method comparison with Mikrogen ELISA, with three lots of ELISA. 64 samples, therefrom 42 negative samples are assayed) It is 100% for all three production lots.
Samples
Serum or plasma
Assay Type
Competitive
Detection Range
0.63 - 60 DU/mL
Intra-assay Precision
The intra-assay (within-run) precision of the Parvovirus B19 IgM ELISA was determined by 20 x measurements of 12 serum samples covering the whole measuring range.
Inter-assay Precision
The inter-assay variation of the Parvovirus B19 IgM ELISA was determined with 3 samples with 2 production kits in 10 independent runs with 2 replicates per run.
Preparation and Storage
2 - 8 degree C
Related Product Information for Parvovirus B19 IgM elisa kit
Intended Uses: Enzyme immunoassay for the determination of IgM-class antibodies to Parvovirus B19 in serum or plasma
Principle of the Assay: The Parvovirus B19 IgM ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids false negative or false positive results. Microtiter wells as a solid phase are coated with Parvovirus B19 antigen. Pretreated samples and ready-for-use controls are pipetted into these wells. During incubation Parvovirus B19-specific antibodies of positive samples and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgM antibodies are dispensed into the wells. During a second incubation this anti-IgM conjugate binds specifically to IgM antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Parvovirus B19-specific IgM antibody in the sample. Absorbance at 450 nm is read using an ELISA microtiter plate reader.
Background/Introduction: The Parvovirus B19 IgM Enzyme Immunoassay Kit provides materials for the determination of IgM-class antibodies to Parvovirus B19 in human serum or plasma.
Principle of the Assay: The Parvovirus B19 IgM ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids false negative or false positive results. Microtiter wells as a solid phase are coated with Parvovirus B19 antigen. Pretreated samples and ready-for-use controls are pipetted into these wells. During incubation Parvovirus B19-specific antibodies of positive samples and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgM antibodies are dispensed into the wells. During a second incubation this anti-IgM conjugate binds specifically to IgM antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Parvovirus B19-specific IgM antibody in the sample. Absorbance at 450 nm is read using an ELISA microtiter plate reader.
Background/Introduction: The Parvovirus B19 IgM Enzyme Immunoassay Kit provides materials for the determination of IgM-class antibodies to Parvovirus B19 in human serum or plasma.
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Product Notes
The Human Parvovirus B19 IgM (Catalog #AAA494186) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494186 ELISA Kit recognizes Human Parvovirus B19 IgM. It is sometimes possible for the material contained within the vial of "Parvovirus B19 IgM, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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