Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human ASAM expression on T98G cells. Cells were stained with PE-conjugated anti-Human ASAM. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Mouse anti-Human ASAM Monoclonal Antibody | anti-CLMP antibody
Anti-ASAM Antibody, Mouse Monoclonal
Gene Names
CLMP; ACAM; ASAM; CSBM; CSBS
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
ASAM; Monoclonal Antibody; Anti-ASAM Antibody; Mouse Monoclonal; ASAM/CLMP Antibody; Mouse MAb; CXADR-like membrane protein; Anti-ACAM Antibody; Anti-CSBM Antibody; Anti-CSBS Antibody; anti-CLMP antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
1H2B5D10
Specificity
Human ASAM/ACAM
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CLMP antibody
ELISA (EIA), Flow Cytometry (FC/FACS/FCM)
Application Notes
ELISA: 1:1000-1:2000
FC/FACS/FCM: 1:25-1:100
FC/FACS/FCM: 1:25-1:100
Immunogen
Recombinant Human ASAM protein
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ASAM extracellular domain (rh ASAM). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human ASAM expression on T98G cells. Cells were stained with PE-conjugated anti-Human ASAM. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human ASAM expression on T98G cells. Cells were stained with PE-conjugated anti-Human ASAM. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Related Product Information for anti-CLMP antibody
Adipocyte-specific adhesion molecule (ASAM), also known as ACAM and CLMP, is a type I transmembrane protein and a member of the CTX (cortical thymocyte marker in Xenopus) family within the immunoglobulin superfamily. ASAM protein is highly expressed in the small intestine and placenta, and is found at intermediate levels in the heart, skeletal muscle, colon, spleen, kidney, and lung, and appears in low levels in the liver and peripheral blood leukocytes as well. ASAM is a transmembrane component of tight junctions in epithelial cells that can mediate cell aggregation and regulate transepithelial resistance across polarized epithelial cells. In addition, its expression is strongly correlated with white adipose tissue (WAT) mass of human and rodents with obesity.
References
Eguchi J, et al. (2005) Identification of adipocyte adhesion molecule (ACAM), a novel CTX gene family, implicated in adipocyte maturation and development of obesity. Biochem J. 387(Pt 2): 343-53.Sze KL, et al. (2008) Expression of CLMP, a novel tight junction protein, is mediated via the interaction of GATA with the Kruppel family proteins, KLF4 and Sp1, in mouse TM4 Sertoli cells. J Cell Physiol. 214(2): 334-44.Sze KL, et al. (2008) Post-transcriptional regulation of CLMP mRNA is controlled by tristetraprolin in response to TNFalpha via c-Jun N-terminal kinase signalling. Biochem J. 410(3): 575-83.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
41,281 Da
NCBI Official Full Name
CXADR-like membrane protein
NCBI Official Synonym Full Names
CXADR-like membrane protein
NCBI Official Symbol
CLMP
NCBI Official Synonym Symbols
ACAM; ASAM; CSBM; CSBS
NCBI Protein Information
CXADR-like membrane protein; CAR-like membrane protein; adipocyte adhesion molecule; adipocyte-specific adhesion molecule; coxsackie- and adenovirus receptor-like membrane protein
UniProt Protein Name
CXADR-like membrane protein
Protein Family
UniProt Gene Name
CLMP
UniProt Synonym Gene Names
ACAM; ASAM; CAR-like membrane protein
UniProt Entry Name
CLMP_HUMAN
NCBI Description
The CTX (see VSIG2, MIM 606011) family of proteins, including ASAM, are type I transmembrane proteins within the Ig superfamily that localize to junctional complexes between endothelial and epithelial cells and may play a role in cell-cell adhesion (Raschperger et al., 2004 [PubMed 14573622]).[supplied by OMIM, Mar 2008]
Uniprot Description
ASAM: May be involved in the cell-cell adhesion. May play a role in adipocyte differentiation and development of obesity.
Protein type: Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q24.1
Cellular Component: cell surface; tight junction; cytoplasmic microtubule; plasma membrane; integral to membrane
Biological Process: gut development
Disease: Congenital Short Bowel Syndrome
Research Articles on CLMP
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Product Notes
The CLMP clmp (Catalog #AAA8103909) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-ASAM Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ASAM can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS/FCM). ELISA: 1:1000-1:2000 FC/FACS/FCM: 1:25-1:100. Researchers should empirically determine the suitability of the CLMP clmp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ASAM, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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