Human Nuclear Protein MDM1 (MDM1) ELISA Kit | MDM1 elisa kit

Human Nuclear Protein MDM1 (MDM1) ELISA Kit

Cat. Num. AAA9715341

• Reactivity: Human
• Synonyms: Nuclear Protein MDM1 (MDM1); Human Nuclear Protein MDM1 (MDM1) ELISA Kit; MDM1; FLJ95264; Mdm4; transformed 3T3 cell double minute 1; p53 binding protein; mouse Mdm1 nuclear protein homolog; nuclear protein MDM1; nuclear protein double minute 1; MDM1 elisa kit

• Reactivity: Human
• Specificity: Human Nuclear protein MDM1 (MDM1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MDM1. No significant cross-reactivity or interference between Human MDM1 and analogues was observed.
• Sequence Length: 679

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Nuclear protein MDM1 (MDM1) Standard Curve.)

Related Product Information for MDM1 elisa kit

Description: This Human Nuclear protein MDM1 (MDM1) ELISA Kit employs a two-site sandwich ELISA to quantitate MDM1 in samples. An antibody specific for MDM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MDM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: MDM1encodes a nuclear protein similar to the mouse double minute 1 protein. The mouse gene is located in double minute (DM) chromatin particles, is amplified in the mouse transformed 3T3 cell line, and the encoded protein is able to bind to p53. Alternatively spliced transcript variants encoding different isoforms have been found for MDM1.

NCBI and Uniprot Product Information

• NCBI GI #: 326381070
• NCBI GeneID: 56890
• NCBI Accession #: NP_001191957.1
• NCBI GenBank Nucleotide #: NP_001191957.1
• UniProt Accession #: Q8TC05
• NCBI Official Full Name: nuclear protein MDM1 isoform 4
• NCBI Official Synonym Full Names: Mdm1 nuclear protein
• NCBI Official Symbol: MDM1
• NCBI Protein Information: nuclear protein MDM1
• UniProt Protein Name: Nuclear protein MDM1
• Protein Family: Nuclear protein
• UniProt Gene Name: MDM1
• UniProt Entry Name: MDM1_HUMAN

NCBI Description

This gene encodes a microtubule-binding nuclear protein that localizes to the centrioles of dividing cells and differentiating multiciliated cells and negatively regulates centriole duplication. The encoded protein is closely associated with the centriole barrel, and resides in the centriole lumen. Naturally-occurring mutations in the orthologous mouse gene are associated with age-related retinal degeneration. [provided by RefSeq, Feb 2019]