Platelet Derived Growth Factor BB (PDGFBB) Recombinant Protein | PDGFBB recombinant protein

Recombinant Platelet Derived Growth Factor BB (PDGFBB)

Cat. Num. AAA2011521

• Gene Names: Pdgfb; SIS; c-sis
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Platelet Derived Growth Factor BB (PDGFBB); Recombinant Platelet Derived Growth Factor BB (PDGFBB); PDGFBB recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-CYLRLV SAEGDPIPEE LYEMLSDHSI RSFDDLQRLL HRDSVDEDGA ELDLNMTRAH SGVESESSSR GRRSLGSLAA AEPAVIAECK TRTEVFQISR NLIDRTNANF LVWPPCVEVQ RCSGCCNNRN VQCRASQVQM RPVQVRKIEI VRKKPVFKKA TVTLEDHLAC KCETVVTPRP VTRSPGTSRE HRAKTPQTRV TVRTVRIRRP PKGKHRKFKH THDKK
• Sequence Length: 241

• Applicable Applications for PDGFBB recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Cys5~Lys225 (Accession # Q05028) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 158081747
• NCBI GeneID: 24628
• NCBI Accession #: NP_113712.1
• NCBI GenBank Nucleotide #: NM_031524.1
• UniProt Accession #: Q05028
• Molecular Weight: 57.1kDa
• NCBI Official Full Name: platelet-derived growth factor subunit B
• NCBI Official Synonym Full Names: platelet-derived growth factor beta polypeptide
• NCBI Official Symbol: Pdgfb
• NCBI Official Synonym Symbols: SIS; c-sis
• NCBI Protein Information: platelet-derived growth factor subunit B; PDGF-2; pdgf protein; PDGF subunit B; platelet-derived growth factor B chain; platelet derived growth factor, B polypeptide; Platelet-derived growth factor, same as Sis (Simian sarcoma viral oncogene homologue, c-sis); platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
• UniProt Protein Name: Platelet-derived growth factor subunit B
• UniProt Gene Name: Pdgfb
• UniProt Synonym Gene Names: PDGF subunit B
• UniProt Entry Name: PDGFB_RAT

NCBI Description

may play a role in carotid artery smooth muscle cell proliferation and neointimal formation in response to balloon catheter injury [RGD, Feb 2006]

Uniprot Description

PDGFB: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB. Belongs to the PDGF/VEGF growth factor family.

Protein type: Oncoprotein; Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Cellular Component: extracellular space; cell surface; membrane; basolateral plasma membrane; cytoplasm

Molecular Function: collagen binding; identical protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; superoxide-generating NADPH oxidase activator activity; chemoattractant activity; receptor binding

Biological Process: positive regulation of cyclin-dependent protein kinase activity; heart development; positive regulation of transcription, DNA-dependent; positive regulation of fibroblast growth factor receptor signaling pathway; cell projection biogenesis; response to organic cyclic substance; protein amino acid phosphorylation; positive regulation of MAP kinase activity; monocyte chemotaxis; positive regulation of fibroblast proliferation; positive chemotaxis; cell growth; positive regulation of mitotic cell cycle, embryonic; response to drug; substrate-bound cell migration; positive regulation of mitosis; positive regulation of chemotaxis; activation of protein kinase B; positive regulation of blood vessel endothelial cell migration; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; response to estrogen stimulus; activation of protein kinase activity; blood vessel morphogenesis; positive regulation of endothelial cell proliferation; actin cytoskeleton organization and biogenesis; negative regulation of transcription, DNA-dependent; embryonic placenta development; peptidyl-tyrosine phosphorylation; wound healing; positive regulation of smooth muscle cell proliferation; platelet-derived growth factor receptor signaling pathway; positive regulation of smooth muscle cell migration; response to estradiol stimulus; response to insulin stimulus; response to organic substance; positive regulation of MAPKKK cascade; positive regulation of glomerular filtration; positive regulation of cell proliferation; response to wounding; hemopoiesis; DNA replication; regulation of peptidyl-tyrosine phosphorylation; negative regulation of cell migration; negative regulation of phosphatidylinositol biosynthetic process; positive regulation of phosphoinositide 3-kinase activity; regulation of cell proliferation; peptidyl-serine phosphorylation; response to hypoxia; blood coagulation; positive regulation of DNA replication; positive regulation of cell migration