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Western Blot (WB) (MDM1 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the MDM1 antibody detected the MDM1 protein (arrow).)

Rabbit anti-Human MDM1 Polyclonal Antibody | anti-MDM1 antibody

MDM1 Antibody (C-term)

Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MDM1; Polyclonal Antibody; MDM1 Antibody (C-term); Nuclear protein MDM1; anti-MDM1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MDM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 656-684 amino acids from the C-terminal region of human MDM1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.42 (varies by lot)
Sequence Positions
656-684
Sequence Length
679
Applicable Applications for anti-MDM1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(MDM1 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the MDM1 antibody detected the MDM1 protein (arrow).)

Western Blot (WB) (MDM1 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the MDM1 antibody detected the MDM1 protein (arrow).)

Immunohistochemistry (IHC)

(MDM1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MDM1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (MDM1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MDM1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-MDM1 antibody
This gene encodes a nuclear protein similar to the mouse
double minute 1 protein. The mouse gene is located in double minute
(DM) chromatin particles and is amplified in the mouse transformed
3T3 cell line, and the protein is able to bind to p53. In mouse
several transcripts have been described for this gene which result
from alternative polyadenylation, splicing and exon usage.
Product Categories/Family for anti-MDM1 antibody
References
Chang, B., et al. Hum. Mol. Genet. 17(24):3929-3941(2008)
Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
Snyder, L.C., et al. J. Biol. Chem. 263(32):17150-17158(1988)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
80735
NCBI Official Full Name
nuclear protein MDM1 isoform 4
NCBI Official Synonym Full Names
Mdm1 nuclear protein
NCBI Official Symbol
MDM1
NCBI Protein Information
nuclear protein MDM1
UniProt Protein Name
Nuclear protein MDM1
UniProt Gene Name
MDM1
UniProt Entry Name
MDM1_HUMAN

NCBI Description

This gene encodes a nuclear protein similar to the mouse double minute 1 protein. The mouse gene is located in double minute (DM) chromatin particles, is amplified in the mouse transformed 3T3 cell line, and the encoded protein is able to bind to p53. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

Uniprot Description

MDM1: a nuclear protein similar to the mouse double minute 1 protein. The mouse gene is located in double minute (DM) chromatin particles, is amplified in the mouse transformed 3T3 cell line, and the encoded protein is able to bind to p53. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 12q15

Cellular Component: nucleus

Biological Process: retina development in camera-type eye

Research Articles on MDM1

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Product Notes

The MDM1 mdm1 (Catalog #AAA9210165) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 656-684. The MDM1 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MDM1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the MDM1 mdm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MDM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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