Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein Mdm4 (MDM4) Standard Curve.)
Mouse Protein Mdm4 (MDM4) ELISA Kit | MDM4 elisa kit
Mouse Protein Mdm4 (MDM4) ELISA Kit
Gene Names
Mdm4; Mdmx; C85810; AA414968; AL023055; AU018793; AU021806; 4933417N07Rik
Reactivity
Mouse
Synonyms
Protein Mdm4 (MDM4); Mouse Protein Mdm4 (MDM4) ELISA Kit; MDM4; RP11-430C7.1; DKFZp781B1423; HDMX; MDMX; MGC132766; MRP1; MDM4-related protein 1; Mdm4; transformed 3T3 cell double minute 4; p53 binding protein; double minute 4; human homolog of; p53-binding protei; MDM4 elisa kit
Reactivity
Mouse
Specificity
Mouse Protein Mdm4 (MDM4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MDM4. No significant cross-reactivity or interference between Mouse MDM4 and analogues was observed.
Sequence Length
489
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein Mdm4 (MDM4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein Mdm4 (MDM4) Standard Curve.)
Related Product Information for MDM4 elisa kit
Description: This Mouse Protein Mdm4 (MDM4) ELISA Kit employs a two-site sandwich ELISA to quantitate MDM4 in samples. An antibody specific for MDM4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MDM4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDM4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDM4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Mdm4 encodes a protein that has been shown to negatively regulate the activity of the tumor suppressor protein p53. Homozygous knockout mice exhibit embryonic lethality as a result of p53-dependent apoptosis and cell cycle arrest. Amplification of Mdm4 or overexpression of the encoded protein has been linked to a range of human cancers. A pseudogene has been identified on the X chromosome. Alternative splicing of Mdm4 results in multiple transcript variants.
Background: Mdm4 encodes a protein that has been shown to negatively regulate the activity of the tumor suppressor protein p53. Homozygous knockout mice exhibit embryonic lethality as a result of p53-dependent apoptosis and cell cycle arrest. Amplification of Mdm4 or overexpression of the encoded protein has been linked to a range of human cancers. A pseudogene has been identified on the X chromosome. Alternative splicing of Mdm4 results in multiple transcript variants.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein Mdm4 isoform 2
NCBI Official Synonym Full Names
transformed mouse 3T3 cell double minute 4
NCBI Official Symbol
Mdm4
NCBI Official Synonym Symbols
Mdmx; C85810; AA414968; AL023055; AU018793; AU021806; 4933417N07Rik
NCBI Protein Information
protein Mdm4
UniProt Protein Name
Protein Mdm4
Protein Family
UniProt Gene Name
Mdm4
UniProt Synonym Gene Names
Mdmx
UniProt Entry Name
MDM4_MOUSE
NCBI Description
This gene encodes a protein that has been shown to negatively regulate the activity of the tumor suppressor protein p53. Homozygous knockout mice exhibit embryonic lethality as a result of p53-dependent apoptosis and cell cycle arrest. Amplification of this gene or overexpression of the encoded protein has been linked to a range of human cancers. A pseudogene has been identified on the X chromosome. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2014]
Research Articles on MDM4
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Product Notes
The Mouse MDM4 mdm4 (Catalog #AAA9717688) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717688 ELISA Kit recognizes Mouse MDM4. It is sometimes possible for the material contained within the vial of "Protein Mdm4 (MDM4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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