Human NOX1 (NADPH oxidase 1) ELISA Kit | NOX1 elisa kit
Human NOX1 (NADPH oxidase 1) ELISA Kit
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NOX1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NOX1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOX1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human NOX1. You can calculate the concentration of Human NOX1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
involved in superoxide generation and serum-stimulated growth [RGD, Feb 2006]
Uniprot Description
NOX1: NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; EC 1.-.-.-; Membrane protein, integral; Channel, misc.; Oxidoreductase; Motility/polarity/chemotaxis
Cellular Component: cytoplasm; early endosome; endosome; NADPH oxidase complex
Molecular Function: protein binding; Rac GTPase binding; superoxide-generating NADPH oxidase activity; voltage-gated proton channel activity
Biological Process: angiogenesis; cell migration; extracellular matrix organization and biogenesis; hydrogen peroxide metabolic process; intracellular pH elevation; positive regulation of cell proliferation; positive regulation of integrin biosynthetic process; positive regulation of JNK cascade; positive regulation of MAPKKK cascade; positive regulation of smooth muscle cell proliferation; proton transport; regulation of systemic arterial blood pressure by renin-angiotensin; response to reactive oxygen species; signal transduction; superoxide metabolic process; superoxide release
Research Articles on NOX1
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Product Notes
The Human NOX1 nox1 (Catalog #AAA2540334) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2540334 ELISA Kit recognizes Human NOX1. It is sometimes possible for the material contained within the vial of "NOX1 (NADPH oxidase 1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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