Immunohistochemistry (IHC)
(Immunochemical staining of human XTP3TPA in human appendix with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)
Rabbit anti-Human XTP3TPA Polyclonal Antibody | anti-DCTPP1 antibody
Anti-XTP3TPA Antibody, Rabbit Polyclonal
ELISA: 1:5000-1:10,000
IHC-P: 1:10,000-1:20,000
IP: 0.5-2uL/mg of lysate
Immunohistochemistry (IHC)
(Immunochemical staining of human XTP3TPA in human appendix with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human XTP3TPA in human appendix with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human XTP3TPA in human kidney with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human XTP3TPA in human kidney with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)
Immunoprecipitation (IP)
(XTP3TPA was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate2 uL anti-XTP3TPA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-XTP3TPA rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size: 22 kDa)
Immunoprecipitation (IP)
(XTP3TPA was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate2 uL anti-XTP3TPA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-XTP3TPA rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size: 22 kDa)
Western Blot (WB)
(Anti-XTP3TPA rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: HEPG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-XTP3TPA rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: HEPG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa(We are unsure as to the identity of these extra bands.))
NCBI and Uniprot Product Information
Uniprot Description
XTP3TPA: Hydrolyzes deoxynucleoside triphosphates (dNTPs) to the corresponding nucleoside monophosphates. Has a strong preference for modified dCTP. Activity is highest with 5-iodo-dCTP, followed by 5-bromo-dCTP, unmodified dCTP, 5-methyl-dCTP and 5-chloro-dCTP. Hydrolyzes 2-chloro-dATP and 2-hydroxy-dATP with lower efficiency, and has even lower activity with unmodified dATP, dTTP and dUTP (in vitro). Does not hydrolyze ATP, UTP, ITP, GTP, dADP, dCDP or dGTP. May protect DNA or RNA against the incorporation of non- canonical nucleotide triphosphates. May protect cells against inappropriate methylation of CpG islands by DNA methyltransferases.
Protein type: EC 3.6.1.12
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: cytosol
Molecular Function: dCTP diphosphatase activity; pyrimidine deoxyribonucleotide binding; magnesium ion binding; nucleoside-triphosphate diphosphatase activity
Biological Process: nucleoside triphosphate catabolic process; protein homotetramerization
Research Articles on DCTPP1
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Product Notes
The DCTPP1 dctpp1 (Catalog #AAA8107435) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-XTP3TPA Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's XTP3TPA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IHC-P: 1:10,000-1:20,000 IP: 0.5-2uL/mg of lysate. Researchers should empirically determine the suitability of the DCTPP1 dctpp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "XTP3TPA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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