Rabbit MOB1A Polyclonal Antibody | anti-MOB1A antibody

MOB1A Antibody

Cat. Num. AAA9611202

• Gene Names: MOB1B; MATS2; MOB4A; MOBKL1A
• Reactivity: Human, Mouse; Predicted: Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: MOB1A; Polyclonal Antibody; MOB1A Antibody; MATS 2; MATS2; MGC33910; Mob 1A; Mob 1B; MOB 4A; MOB kinase activator 1B; Mob1 homolog 1A; MOB1 Mps One Binder homolog B; MOB1 Mps one binder kinase activator like 1A; Mob1A; Mob1B; MOBKL 1A; MOBKL1A; MOL1A_HUMAN; Mps one binder kinase activator like 1A; Mps one binder kinase activator-like 1A; Protein Mob4A; anti-MOB1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted: Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MOB1A Antibody detects endogenous levels of MOB1A.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 221

• Applicable Applications for anti-MOB1A antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA (Peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human MOB1A.
• Tissue Specificity: Adrenal gland, bone marrow, brain, lung, placenta, prostate, salivary gland, skeletal muscle, testis, thymus, thyroid gland, uterus, colon with mucosa, fetal brain and fetal liver.
• Subcellular Location: Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of MOB1A in lysates of 293, using MOB1A Antibody.)

Related Product Information for anti-MOB1A antibody

Description: Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38L.

Post Translational Modifications: Phosphorylated by STK3/MST2 and STK4/MST1 and this phosphorylation enhances its binding to LATS1.

Subunit Structure: Binds STK38L. Interacts with LATS1 and LATS2.

Similarity: Belongs to the MOB1/phocein family.

NCBI and Uniprot Product Information

• NCBI GI #: 348041379
• NCBI GeneID: 92597
• NCBI Accession #: NP_001231695.1
• NCBI GenBank Nucleotide #: NP_001231695.1
• UniProt Accession #: Q7L9L4
• Molecular Weight: Observed: 25 kDa; Predicted: 26 kDa
• NCBI Official Full Name: MOB kinase activator 1B isoform 1
• NCBI Official Synonym Full Names: MOB kinase activator 1B
• NCBI Official Symbol: MOB1B
• NCBI Official Synonym Symbols: MATS2; MOB4A; MOBKL1A
• NCBI Protein Information: MOB kinase activator 1B
• UniProt Protein Name: MOB kinase activator 1B
• Protein Family: MOB kinase activator
• UniProt Gene Name: MOB1B
• UniProt Synonym Gene Names: MOB4A; MOBKL1A; Mob1A
• UniProt Entry Name: MOB1B_HUMAN

NCBI Description

The protein encoded by this gene is similar to the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

MOB1B: Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38L. Binds STK38L. Interacts with LATS1 and LATS2. Adrenal gland, bone marrow, brain, lung, placenta, prostate, salivary gland, skeletal muscle, testis, thymus, thyroid gland, uterus, colon with mucosa, fetal brain and fetal liver. Belongs to the MOB1/phocein family.

Protein type: Activator; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: protein binding; metal ion binding; kinase activator activity; kinase binding

Biological Process: protein amino acid autophosphorylation; positive regulation of phosphorylation