Human Neutrophil Cytosolic Factor 1 (NCF1) ELISA Kit | NCF1 elisa kit

Human Neutrophil Cytosolic Factor 1 (NCF1) ELISA Kit

Cat. Num. AAA2000375

• Gene Names: NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A
• Reactivity: Human
• Synonyms: Neutrophil Cytosolic Factor 1 (NCF1); Human Neutrophil Cytosolic Factor 1 (NCF1) ELISA Kit; NCF1A; NOXO2; SH3PXD1A; p47-phox; NCF-47K; 47 kDa autosomal chronic granulomatous disease protein; Neutrophil NADPH oxidase factor 1; Nox-organizing protein 2; NCF1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Neutrophil Cytosolic Factor 1 (NCF1).; No significant cross-reactivity or interference between Neutrophil Cytosolic Factor 1 (NCF1) and analogues was observed.
• Sequence Length: 390

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.113ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neutrophil Cytosolic Factor 1 (NCF1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neutrophil Cytosolic Factor 1 (NCF1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NCF1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutrophil Cytosolic Factor 1 (NCF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neutrophil Cytosolic Factor 1 (NCF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neutrophil Cytosolic Factor 1 (NCF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neutrophil Cytosolic Factor 1 (NCF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for NCF1 elisa kit

Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 528078313
• NCBI GeneID: 653361
• NCBI Accession #: NP_000256.4
• NCBI GenBank Nucleotide #: NM_000265.5
• UniProt Accession #: P14598; O43842; Q2PP07; Q53FR5; Q9BU90; Q9BXI7; Q9BXI8; Q9UDV9; Q9UMU2; A6NEH2; A8K7S9
• Molecular Weight: 30,781 Da
• NCBI Official Full Name: neutrophil cytosol factor 1
• NCBI Official Synonym Full Names: neutrophil cytosolic factor 1
• NCBI Official Symbol: NCF1
• NCBI Official Synonym Symbols: NCF1A; NOXO2; p47phox; SH3PXD1A
• NCBI Protein Information: neutrophil cytosol factor 1
• UniProt Protein Name: Neutrophil cytosol factor 1
• Protein Family: Neutrophil cytosol factor
• UniProt Gene Name: NCF1
• UniProt Synonym Gene Names: NOXO2; SH3PXD1A; NCF-1
• UniProt Entry Name: NCF1_HUMAN

NCBI Description

The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008]

Uniprot Description

p47phox: the 47-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. The holo-oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Contains 2 SH2 domains. Mutations in NCF1, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease.

Protein type: EC 1.-.-.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: Golgi apparatus; extrinsic to membrane; rough endoplasmic reticulum; cell soma; dendrite; cytosol; NADPH oxidase complex

Molecular Function: protein binding; electron carrier activity; superoxide-generating NADPH oxidase activity; phosphatidylinositol-3,4-bisphosphate binding; SH3 domain binding; phosphoinositide binding

Biological Process: respiratory burst during defense response; respiratory burst; interaction with host; apoptosis; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; leukotriene metabolic process; cell proliferation; antigen processing and presentation of peptide antigen via MHC class I; negative regulation of smooth muscle contraction; innate immune response; cellular defense response; antigen processing and presentation of exogenous peptide antigen via MHC class I; response to yeast; vascular endothelial growth factor receptor signaling pathway; protein targeting to membrane; inflammatory response; superoxide release; hydrogen peroxide biosynthetic process

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type I