Rabbit anti-Human PRF1 Polyclonal Antibody | anti-PRF1 antibody

PRF1 Antibody

Cat. Num. AAA7004858

• Gene Names: PRF1; P1; PFP; FLH2; PFN1; HPLH2
• Reactivity: Human
• Applications: ELISA
• Purity: >95%,Protein G purified
• Synonyms: PRF1; Polyclonal Antibody; PRF1 Antibody; Perforin-1; P1; Cytolysin; Lymphocyte pore-forming protein; PFP; anti-PRF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%,Protein G purified
• Form/Format: Liquid
• Sequence Length: 555

• Applicable Applications for anti-PRF1 antibody: ELISA (EIA)
• Immunogen: Recombinant human Perforin-1 protein
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western BlotPositive WB detected in: HepG2 whole cell lysateAll lanes: Prf1 antibody at 4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 62 KDaObserved band size: 62 KDa)

Related Product Information for anti-PRF1 antibody

Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.

NCBI and Uniprot Product Information

• NCBI GI #: 133908621
• NCBI GeneID: 5551
• NCBI Accession #: NP_001076585.1
• NCBI GenBank Nucleotide #: NM_001083116.1
• UniProt Accession #: P14222; Q59F57; Q86WX7; B2R6X4
• Molecular Weight: 61,377 Da
• NCBI Official Full Name: perforin-1
• NCBI Official Synonym Full Names: perforin 1
• NCBI Official Symbol: PRF1
• NCBI Official Synonym Symbols: P1; PFP; FLH2; PFN1; HPLH2
• NCBI Protein Information: perforin-1
• UniProt Protein Name: Perforin-1
• Protein Family: Perforin
• UniProt Gene Name: PRF1
• UniProt Synonym Gene Names: PFP; P1; PFP
• UniProt Entry Name: PERF_HUMAN

NCBI Description

The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

PRF1: Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. Monomer, as sobluble protein. Homooligomer. Oligomerization is required for pore formation. Repressed by contact with target cells. Belongs to the complement C6/C7/C8/C9 family.

Protein type: Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: cytoplasmic membrane-bound vesicle; extracellular region; integral to membrane; membrane; plasma membrane

Molecular Function: calcium ion binding; protein binding; wide pore channel activity

Biological Process: apoptosis; cellular defense response; cytolysis; defense response to tumor cell; defense response to virus; formation of immunological synapse; immune response to tumor cell; protein homooligomerization; transmembrane transport

Disease: Aplastic Anemia; Hemophagocytic Lymphohistiocytosis, Familial, 2; Lymphoma, Non-hodgkin, Familial