Cyclin D3 Recombinant Protein | CCND3 recombinant protein

Recombinant Cyclin D3 (CCND3)

Cat. Num. AAA2029082

• Applications: SDS-Page, Western Blot
• Purity: >80%
• Synonyms: Cyclin D3; Recombinant Cyclin D3 (CCND3); CCND3 recombinant protein

• Host: E. coli
• Purity/Purification: >80%
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
• Concentration: Original Concentration: 400ug/mL (varies by lot)
• Sequence: MELLCCEGTR HAPRAGPDPR LLGDQRVLQS LLRLEERYVP RASYFQCVQR EIKPHMRKML AYWMLEVCEE QRCEEEVFPL AMNYLDRYLS CVPTRKAQLQ LLGAVCMLLA SKLRETTPLT IEKLCIYTDH AVSPRQLRDW EVLVLGKLKW DLAAVIAHDF LAFILHRLSL PRDRQALVKK HAQTFLALCA TDYTFAMYPP SMIATGSIGA AVQGLGACSM SGDELTELLA GITGTEVDCLRACQEQIEAA LRESLREASQ TSSSPAPKAP RGSSSQGPSQ TSTPTDVTAI HL

• Applicable Applications for CCND3 recombinant protein: Positive Control; Immunogen; SDS-PAGE; Western Blot (WB); ; (May be suitable for use in other assays to be determined by the end user.)
• Organism Species: Homo sapiens (Human)
• Source: Prokaryotic expression
• Residues: Met1~Leu292
• Tags: N-terminal His Tag
• Subcellular Location: Nucleus, Chromosome
• Traits: Freeze-dried powder
• Predicted isoelectric point: 7.2
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles.; Store at 2-8ºC for one month.; Aliquot and store at -80ºC for 12 months. ; ; Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 181247
• NCBI GeneID: 896
• Molecular Weight: Predicted Molecular Mass: 36.2kDa; Accurate Molecular Mass: 36kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: cyclin D3
• NCBI Official Synonym Full Names: cyclin D3
• NCBI Official Symbol: CCND3
• NCBI Protein Information: G1/S-specific cyclin-D3
• UniProt Protein Name: G1/S-specific cyclin-D3
• Protein Family: G1/S-specific cyclin
• UniProt Gene Name: CCND3
• UniProt Entry Name: CCND3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

CCND3: Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/p27Kip1, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Interacts with ATF5. Interacts with EIF3K. Component of the ternary complex cyclin D/CDK4/p27Kip1 required for nuclear translocation and modulation of CDK4-mediated kinase activity. Can form similar complexes with either p21Cip1 or CDKN2A. Belongs to the cyclin family. Cyclin D subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Cell cycle regulation; Activator

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: membrane; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleus

Molecular Function: protein binding; cyclin-dependent protein kinase activity; protein kinase binding

Biological Process: T cell proliferation; positive regulation of cyclin-dependent protein kinase activity; cell division; positive regulation of protein amino acid phosphorylation; signal transduction; cell cycle