Human Neuroplastin (NPTN) ELISA Kit | NPTN elisa kit

Human Neuroplastin (NPTN) ELISA Kit

Cat. Num. AAA456283

• Gene Names: NPTN; GP55; GP65; SDR1; np55; np65; SDFR1
• Reactivity: Human
• Synonyms: Neuroplastin (NPTN); Human Neuroplastin (NPTN) ELISA Kit; NPTN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NPTN. No significant cross-reactivity or interference between NPTN and analogues was observed.
• Sequence Length: 394

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.052ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NPTN were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NPTN were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NPTN elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NPTN in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NPTN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NPTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NPTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NPTN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 238624147
• NCBI GeneID: 27020
• NCBI Accession #: NP_001154835.1
• NCBI GenBank Nucleotide #: NM_001161363.1
• UniProt Accession #: Q9Y639; Q17R52; Q59EJ9; Q6NVX7; Q9Y640; B2RAL7; B7Z4D3; B7ZLL2
• Molecular Weight: 43,931 Da
• NCBI Official Full Name: neuroplastin isoform c
• NCBI Official Synonym Full Names: neuroplastin
• NCBI Official Symbol: NPTN
• NCBI Official Synonym Symbols: GP55; GP65; SDR1; np55; np65; SDFR1
• NCBI Protein Information: neuroplastin
• UniProt Protein Name: Neuroplastin
• Protein Family: Neuroplastin
• UniProt Gene Name: NPTN
• UniProt Synonym Gene Names: SDFR1; SDR1; SDR-1

NCBI Description

This gene encodes a type I transmembrane protein belonging to the Ig superfamily. The protein is believed to be involved in cell-cell interactions or cell-substrate interactions. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2009]

Uniprot Description

Probable homophilic and heterophilic cell adhesion molecule involved in long term potentiation at hippocampal excitatory synapses through activation of p38MAPK. May also regulate neurite outgrowth by activating the FGFR1 signaling pathway. May play a role in synaptic plasticity ().