Goat SYT1 / Synaptotagmin Polyclonal Antibody | anti-SYT1 antibody

Goat Polyclonal to Human SYT1 / Synaptotagmin

Cat. Num. AAA247728

• Gene Names: SYT1; P65; SYT; SVP65
• Reactivity: Bat, Gibbon, Bovine, Chicken, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Pig, Rabbit, Rat, Zebrafish; Predicted Reactivity: Xenopus (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: SYT1 / Synaptotagmin; Polyclonal Antibody; Goat Polyclonal to Human SYT1 / Synaptotagmin; Anti-SYT1 / Synaptotagmin Antibody (Internal) IHC-plus; SYT1; SYT; SVP65; Synaptotagmin; Synaptotagmin 1; SytI; Synaptotagmin I; Synaptotagmin-1; Human SYT1; anti-SYT1 antibody

• Host: Goat
• Reactivity: Bat, Gibbon, Bovine, Chicken, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Pig, Rabbit, Rat, Zebrafish; Predicted Reactivity: Xenopus (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human SYT1 / Synaptotagmin. Reported isoforms NP_005630.1, NP_001129277.1 and NP_001129278.1 represent identical protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 422

• Applicable Applications for anti-SYT1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:2000), IHC-P (3.75 ug/ml), WB (0.3 - 1 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:2000. Western blot: Approx. 65kDa band observed in Human Brain (hippocampus) and Mouse and Rat Brain lysates (calculated MW of 47.6kDa according to NP_005630.1). The observed molecular weight corresp...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-RDLQSAEKEEQEK from an internal region of human SYT1 / Synaptotagmin (NP_005630.1).
• Immunogen Type: Synthetic peptide
• Immunogen: SYT1 / Synaptotagmin antibody was raised against synthetic peptide C-RDLQSAEKEEQEK from an internal region of human SYT1 / Synaptotagmin (NP_005630.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard, Pufferfish, Zebrafish (100%); Xenopus, Stickleback (92%); Seabass, Magnaporthe (85%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-SYT1 / Synaptotagmin antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Western Blot (WB)

(SYT1 antibody (1 ug/ml) staining of human hippocampus lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Product Categories/Family for anti-SYT1 antibody

Family: Synaptotagmin

NCBI and Uniprot Product Information

• NCBI GI #: 5032139
• NCBI GeneID: 6857
• NCBI Accession #: NP_005630.1
• NCBI GenBank Nucleotide #: NM_005639.2
• UniProt Accession #: P21579; Q6AI31
• Molecular Weight: 47,573 Da
• NCBI Official Full Name: synaptotagmin-1 isoform 1
• NCBI Official Synonym Full Names: synaptotagmin I
• NCBI Official Symbol: SYT1
• NCBI Official Synonym Symbols: P65; SYT; SVP65
• NCBI Protein Information: synaptotagmin-1; sytI; synaptotagmin 1
• UniProt Protein Name: Synaptotagmin-1
• Protein Family: Synaptotagmin
• UniProt Gene Name: SYT1
• UniProt Synonym Gene Names: SVP65; SYT; SytI
• UniProt Entry Name: SYT1_HUMAN

NCBI Description

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010]

Uniprot Description

SYT1: an integral membrane protein of synaptic vesicles thought to serve as a Ca(2+) sensor in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse. Binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.

Protein type: Membrane protein, integral; Vesicle; Calcium-binding

Chromosomal Location of Human Ortholog: 12cen-q21

Cellular Component: SNARE complex; presynaptic membrane; synaptic vesicle; neuron projection; synaptic vesicle membrane; integral to membrane; plasma membrane; excitatory synapse; cell junction

Molecular Function: protein C-terminus binding; identical protein binding; clathrin binding; calcium-dependent phospholipid binding; transporter activity; calcium ion binding; calcium-dependent protein binding; calmodulin binding; phosphatidylinositol-4,5-bisphosphate binding; SNARE binding; protein binding; low-density lipoprotein receptor binding; phosphatidylserine binding; syntaxin-1 binding; phosphatidylinositol binding

Biological Process: regulation of exocytosis; synaptic transmission; positive regulation of calcium ion-dependent exocytosis; synaptic vesicle exocytosis; regulation of synaptic transmission, glutamatergic; vesicle docking; glutamate secretion; neurotransmitter secretion; positive regulation of vesicle fusion; pathogenesis; detection of calcium ion; positive regulation of synaptic transmission; protein homooligomerization