Human Neuropeptide Y (NPY) ELISA Kit | NPY elisa kit

Human Neuropeptide Y (NPY) ELISA Kit

Cat. Num. AAA2700376

• Gene Names: NPY; PYY4
• Reactivity: Human
• Synonyms: Neuropeptide Y (NPY); Human Neuropeptide Y (NPY) ELISA Kit; NP-Y; PYY4; CPON; Pro-neuropeptide Y; Neuropeptide tyrosine; C-flanking peptide of NPY; NPY elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Neuropeptide Y (NPY).; No significant cross-reactivity or interference between Neuropeptide Y (NPY) and analogues was observed.
• Sequence Length: 97

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 24.69-2000pg/mL
• Sensitivity: < 9.18pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuropeptide Y (NPY) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuropeptide Y (NPY) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NPY elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neuropeptide Y (NPY) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neuropeptide Y (NPY) and unlabeled Neuropeptide Y (NPY) (Standards or samples) with the pre-coated antibody specific to Neuropeptide Y (NPY). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neuropeptide Y (NPY) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Neuropeptide Y (NPY) in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 386984
• NCBI GeneID: 4852
• UniProt Accession #: P01303
• Molecular Weight: 10,851 Da
• NCBI Official Full Name: neuropeptide Y, partial
• NCBI Official Synonym Full Names: neuropeptide Y
• NCBI Official Symbol: NPY
• NCBI Official Synonym Symbols: PYY4
• NCBI Protein Information: pro-neuropeptide Y
• UniProt Protein Name: Pro-neuropeptide Y
• Protein Family: Neuropeptide
• UniProt Gene Name: NPY
• UniProt Synonym Gene Names: NPY; CPON

NCBI Description

This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases. The protein also exhibits antimicrobial activity against bacteria and fungi. [provided by RefSeq, Oct 2014]

Uniprot Description

NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.