Mouse Neuropeptide Y (NPY) ELISA Kit | NPY elisa kit
Mouse Neuropeptide Y (NPY) ELISA Kit
Gene Names
NPY; PYY4
Reactivity
Mouse
Synonyms
Neuropeptide Y (NPY); Mouse Neuropeptide Y (NPY) ELISA Kit; NP-Y; PYY4; CPON; Pro-neuropeptide Y; Neuropeptide tyrosine; C-flanking peptide of NPY; NPY elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of NPY. No significant cross-reactivity or interference between NPY and analogues was observed.
Sequence Length
97
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type
Quantitative Competitive
Detection Range
2.47-200pg/mL
Sensitivity
< 0.97pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NPY were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NPY were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NPY elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of NPY in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to NPY has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled NPY and unlabeled NPY (Standards or samples) with the pre-coated antibody specific to NPY. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of NPY in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of NPY in the sample.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to NPY has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled NPY and unlabeled NPY (Standards or samples) with the pre-coated antibody specific to NPY. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of NPY in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of NPY in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
10,851 Da
NCBI Official Full Name
neuropeptide Y, partial
NCBI Official Synonym Full Names
neuropeptide Y
NCBI Official Symbol
NPY
NCBI Official Synonym Symbols
PYY4
NCBI Protein Information
pro-neuropeptide Y
UniProt Protein Name
Pro-neuropeptide Y
Protein Family
UniProt Gene Name
NPY
UniProt Synonym Gene Names
NPY; CPON
NCBI Description
This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases. The protein also exhibits antimicrobial activity against bacteria and fungi. [provided by RefSeq, Oct 2014]
Uniprot Description
NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
