Rat DKK-1 ELISA Kit | DKK-1 elisa kit

Rat DKK-1 PicoKine Quick ELISA Kit

Cat. Num. AAA128094

• Reactivity: Rat
• Applications: ELISA
• Synonyms: DKK-1; N/A; Rat DKK-1 PicoKine Quick ELISA Kit; Dickkopf homolog 1; dickkopf WNT signaling pathway inhibitor 1; Dickkopf homolog 1 (Xenopus laevis)(Predicted) ; Dickkopf-like protein 1 ; Protein Dkk1 ; Dkk1 ; DKK1 , Dkk1_predicted , LOC100912050 ; rCG_48380; DKK-1 elisa kit

• Reactivity: Rat
• Samples: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Assay Range: 46.9pg/ml-3,000pg/ml
• Sensitivity: <15pg/ml
• Capture antibody and Detection antibody: monoclonal antibody from mouse|polyclonal antibody from goat
• Immunogen: Expression system for standard: NS0; Immunogen sequence: S23-H270
• Anticoagulant: heparin or EDTA
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

• Applicable Applications for DKK-1 elisa kit: ELISA
• Kit Components: Description/Quantity; Pre-coated 96-well strip microplate/1; Standard/2 vials, 10 ng/tube; Biotinylated antibody (100x)/100ul; Avidin-Biotin-Peroxidase Complex (100x)/100ul; Sample Diluent/30ml; Antibody Diluent/12ml; Avidin-Biotin-Peroxidase Diluent/12ml; Color Developing Reagent (TMB)/10ml; Stop Solution/10ml; Wash Buffer (25x)/20ml; Adhesive plate sealers/4
• Materials Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm.; Incubator.; Automated plate washer (optional).; Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.; Multichannel pipettes are recommended for large amount of samples.; Deionized or distilled water.; 500ml graduated cylinders.; Test tubes for dilution.
• Sample Data:

  Concentration (pg/ml)	0	46.9	93.8	187.5	375	750	1500	3000
  O.D.	0.010	0.089	0.136	0.243	0.464	0.862	1.376	2.000

• Intra Assay Consistency & Inter Assay Consistency:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/ml)	91	361	1121	84	359	1024
  Standard deviation	6.46	28.51	88.4%9	7.39	38.4%8	86.01
  CV (%)	7.1%	7.9%	7.6%	8.8%	9.8%	8.4%

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	91	93	93	92	92	0.82	0.8%
  Sample 2	361	310	365	327	340	23.09	6.7%
  Sample 3	1121	1338	1162	1204	1206	81.53	6.7%

  *number of samples for each test n=16.
• Preparation and Storage: Store at 4 degree C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Standard Curve (Sample)

(Rat DKK1 PicoKine ELISA Kit standard curve)

Related Product Information for DKK-1 elisa kit

The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Categories/Family for DKK-1 elisa kit

Quick ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 157822129
• NCBI GeneID: 293897
• NCBI Accession #: NP_001099820.1
• NCBI GenBank Nucleotide #: NM_001106350.1
• UniProt Accession #: D3Z9J1
• Molecular Weight: 29,149 Da
• NCBI Official Full Name: dickkopf-related protein 1
• NCBI Official Synonym Full Names: dickkopf WNT signaling pathway inhibitor 1
• NCBI Official Symbol: Dkk1
• NCBI Protein Information: dickkopf-related protein 1
• UniProt Protein Name: Dickkopf homolog 1 (Xenopus laevis) (Predicted)
• UniProt Gene Name: Dkk1
• UniProt Entry Name: D3Z9J1_RAT