Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Nesprin-2 (SYNE2) Standard Curve.)
Human Nesprin-2 (SYNE2) ELISA Kit | SYNE2 elisa kit
Human Nesprin-2 (SYNE2) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Nesprin-2 (SYNE2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Nesprin-2 (SYNE2) Standard Curve.)
Background: SYNE2 is a nuclear outer membrane protein that binds cytoplasmic F-actin. This binding tethers the nucleus to the cytoskeleton and aids in the maintenance of the structural integrity of the nucleus. Several transcript variants encoding different isoforms have been found for this gene. SYNE2 contains multiple spectrin repeats and a 60-amino acid C-terminal region homologous to the Drosophila protein Klarsicht. RT-PCR analysis showed low to moderate expression of 2 SYNE2 splice variants in most tissues examined, including skeletal and cardiac muscle, kidney, liver, pancreas, and placenta, and indicated that alternative splicing patterns are conserved between SYNE1 and SYNE2.Nesprin-2-gamma also has a bipartite nuclear localization signal and a leucine zipper motif.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a nuclear outer membrane protein that binds cytoplasmic F-actin. This binding tethers the nucleus to the cytoskeleton and aids in the maintenance of the structural integrity of the nucleus. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Uniprot Description
SYNE2: a nuclear transmembrane protein involved in the maintenance of nuclear organization and structural integrity. The largest part of the protein is cytoplasmic, while its C-terminal part is associated with the nuclear envelope, most probably the outer nuclear membrane. Remains associated with the nuclear envelope during its breakdown in mitotic cells. Probable anchoring protein which theters the nucleus to the cytoskeleton. Connects nuclei to the cytoskeleton by interacting with the nuclear envelope and with F-actin in the cytoplasm. Nine alternatively spliced isoforms have been described.
Protein type: Membrane protein, integral; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 14q23.2
Cellular Component: nuclear outer membrane; filopodium membrane; intermediate filament cytoskeleton; sarcoplasmic reticulum membrane; nuclear membrane; focal adhesion; nuclear lumen; mitochondrion; sarcoplasmic reticulum; integral to membrane; nuclear envelope; Z disc; nucleoplasm; cytoplasm; nucleus
Molecular Function: actin filament binding; protein binding; actin binding
Biological Process: centrosome localization; establishment and/or maintenance of cell polarity; nuclear migration along microfilament; nuclear migration; nuclear membrane organization and biogenesis; positive regulation of cell migration
Disease: Emery-dreifuss Muscular Dystrophy 5, Autosomal Dominant
Research Articles on SYNE2
Similar Products
Product Notes
The Human SYNE2 syne2 (Catalog #AAA9714081) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714081 ELISA Kit recognizes Human SYNE2. It is sometimes possible for the material contained within the vial of "Nesprin-2 (SYNE2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
