Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Nesprin-2 (SYNE2) Standard Curve.)
Mouse Nesprin-2 (SYNE2) ELISA Kit | SYNE2 elisa kit
Mouse Nesprin-2 (SYNE2) ELISA Kit
Gene Names
Syne2; NUA; dice; Cpfl8; KASH2; Nesp2g; AW546258; mKIAA1011; D12Ertd777e; 6820443O06Rik
Reactivity
Mouse
Synonyms
Nesprin-2 (SYNE2); Mouse Nesprin-2 (SYNE2) ELISA Kit; DKFZp434H2235; DKFZp686E01115; DKFZp686H1931; EDMD5; FLJ11014; FLJ43727; FLJ45710; FLJ46790; KIAA1011; NUA; NUANCE; Nesprin-2; SYNE-2; nesprin 2; nucleus and actin connecting element; synaptic nuclei; SYNE2 elisa kit
Reactivity
Mouse
Specificity
Mouse Nesprin-2 (SYNE2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SYNE2. No significant cross-reactivity or interference between Mouse SYNE2 and analogues was observed.
Sequence Length
6874
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Nesprin-2 (SYNE2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Nesprin-2 (SYNE2) Standard Curve.)
Related Product Information for SYNE2 elisa kit
Description: This Mouse Nesprin-2 (SYNE2) ELISA Kit employs a two-site sandwich ELISA to quantitate SYNE2 in samples. An antibody specific for SYNE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SYNE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYNE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SYNE2 is a nuclear outer membrane protein that binds cytoplasmic F-actin. This binding tethers the nucleus to the cytoskeleton and aids in the maintenance of the structural integrity of the nucleus. Several transcript variants encoding different isoforms have been found for this gene. SYNE2 contains multiple spectrin repeats and a 60-amino acid C-terminal region homologous to the Drosophila protein Klarsicht. RT-PCR analysis showed low to moderate expression of 2 SYNE2 splice variants in most tissues examined, including skeletal and cardiac muscle, kidney, liver, pancreas, and placenta, and indicated that alternative splicing patterns are conserved between SYNE1 and SYNE2.Nesprin-2-gamma also has a bipartite nuclear localization signal and a leucine zipper motif.
Background: SYNE2 is a nuclear outer membrane protein that binds cytoplasmic F-actin. This binding tethers the nucleus to the cytoskeleton and aids in the maintenance of the structural integrity of the nucleus. Several transcript variants encoding different isoforms have been found for this gene. SYNE2 contains multiple spectrin repeats and a 60-amino acid C-terminal region homologous to the Drosophila protein Klarsicht. RT-PCR analysis showed low to moderate expression of 2 SYNE2 splice variants in most tissues examined, including skeletal and cardiac muscle, kidney, liver, pancreas, and placenta, and indicated that alternative splicing patterns are conserved between SYNE1 and SYNE2.Nesprin-2-gamma also has a bipartite nuclear localization signal and a leucine zipper motif.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
nesprin-2
NCBI Official Synonym Full Names
spectrin repeat containing, nuclear envelope 2
NCBI Official Symbol
Syne2
NCBI Official Synonym Symbols
NUA; dice; Cpfl8; KASH2; Nesp2g; AW546258; mKIAA1011; D12Ertd777e; 6820443O06Rik
NCBI Protein Information
nesprin-2
UniProt Protein Name
Nesprin-2
Protein Family
UniProt Gene Name
Syne2
UniProt Synonym Gene Names
Protein NUANCE; Syne-2
UniProt Entry Name
SYNE2_MOUSE
Research Articles on SYNE2
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Product Notes
The Mouse SYNE2 syne2 (Catalog #AAA9716896) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716896 ELISA Kit recognizes Mouse SYNE2. It is sometimes possible for the material contained within the vial of "Nesprin-2 (SYNE2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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