HRAS-like suppressor3 Recombinant Protein | PLA2G16 recombinant protein

HRAS-like suppressor3,1-133aa, Human,E Coli

Cat. Num. AAA203247

• Gene Names: PLA2G16; AdPLA; HRSL3; HRASLS3; HREV107; H-REV107; HREV107-1; HREV107-3; H-REV107-1
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: HRAS-like suppressor3; 1-133aa; Human; E Coli; HRASLS3; PLA2G16; AdPLA; H-REV107-1; HREV107; HREV107-3; MGC118754; HRAS-like suppressor 3; H rev 107 protein homolog; HRAS like suppressor 3; HREV107 3; Renal carcinoma antigen NY REN 65; ; PLA2G16 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0)
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MRAPIPEPKP GDLIEIFRPF YRHWAIYVGD GYVVHLAPPS EVAGAGAASV MSALTDKAIV KKELLYDVAG SDKYQVNNKH DDKYSPLPCS KIIQRAEELV GQEVLYKLTS ENCEHFVNEL RYGVARSDQV RDV
• Sequence Length: 162

• Applicable Applications for PLA2G16 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PLA2G16 recombinant protein

The HRAS like-suppressor 3 (HRASLS3) belongs to a class II tumor suppressor gene family and is involved in the regulation of differentiation and survival. This protein is expressed in several human tumors including ovarian carcinomas, lung carcinomas and may be involved in interferon-dependent cell death. Recombinant HRAS-like suppressor 3 was expressed in E Coli and was purified by conventional chromatography techniques.

Product Categories/Family for PLA2G16 recombinant protein

Cancer

References

Nazarenko I., et al. (2006) Am. J. Pathol. 169 (4): 1427-39.; Nazarenko I.,et al. (2007) J. Cell. Sci. 120: 1393-404.;

NCBI and Uniprot Product Information

• NCBI GI #: 189571621
• NCBI GeneID: 11145
• NCBI Accession #: NP_001121675
• NCBI GenBank Nucleotide #: NM_001128203.1
• UniProt Accession #: P53816; Q3SYI3; Q9HDD1; B2R7Q4; B7XAK5
• Molecular Weight: 14.9 kDa (133 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: HRAS-like suppressor 3
• NCBI Official Synonym Full Names: phospholipase A2 group XVI
• NCBI Official Symbol: PLA2G16
• NCBI Official Synonym Symbols: AdPLA; HRSL3; HRASLS3; HREV107; H-REV107; HREV107-1; HREV107-3; H-REV107-1
• NCBI Protein Information: HRAS-like suppressor 3
• UniProt Protein Name: HRAS-like suppressor 3
• Protein Family: HRAS-like suppressor
• UniProt Gene Name: PLA2G16
• UniProt Synonym Gene Names: HREV107-1

Uniprot Description

Lipid-modifying enzyme that acts as major regulator of adipocyte lipolysis by catalyzing the release of fatty acids from phospholipids in adipose tissue (PubMed:19615464, PubMed:19047760, PubMed:20837014, PubMed:22605381, PubMed:22923616). Shows phospholipase A1 and A2 activity, catalyzing the calcium-independent hydrolysis of acyl groups in various phosphatidylcholines (PC) and phosphatidylethanolamine (PE) (PubMed:19615464, PubMed:19047760, PubMed:20837014, PubMed:22605381, PubMed:22923616). For most substrates, phospholipase A1 activity is much higher than phospholipase A2 activity (PubMed:19047760). Phospholipase activity causes decreased intracellular levels of ether-type lipids, affecting peroxisome metabolism (). May also have acyltransferase activity: catalyzes both N-acylation of phosphatidylethanolamine to form N-acyl-phosphatidylethanolamine and O-acylation of lyso-phosphatidylcholines to form phosphatidylcholines (PubMed:22605381, PubMed:25383759). The relevance of acyltransferase activity in vivo is however unclear and would require additional evidences (PubMed:22605381, PubMed:25383759). Also has weak lysophospholipase activity ().