Human Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B) ELISA Kit | MYH7B elisa kit

Human Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B) ELISA Kit

Cat. Num. AAA2707156

• Gene Names: MYH7B; MHC14; MYH14
• Reactivity: Human
• Synonyms: Myosin Heavy Chain 7B; Cardiac Muscle Beta (MYH7B); Human Myosin Heavy Chain 7B; Cardiac Muscle Beta (MYH7B) ELISA Kit; MYH14; MHC14; Antigen MLAA-21; Myosin cardiac muscle beta chain; MYH7B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B).; No significant cross-reactivity or interference between Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B) and analogues was observed.
• Sequence Length: 174

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MYH7B elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 153217512
• NCBI GeneID: 57644
• UniProt Accession #: A7E2Y1; Q5JVW7; Q6NT44; Q6NT57; Q6WG75; Q96I57; Q9NWE2; Q9P216
• Molecular Weight: 20,273 Da
• NCBI Official Full Name: Myosin, heavy chain 7B, cardiac muscle, beta
• NCBI Official Synonym Full Names: myosin heavy chain 7B
• NCBI Official Symbol: MYH7B
• NCBI Official Synonym Symbols: MHC14; MYH14
• NCBI Protein Information: myosin-7B
• UniProt Protein Name: Myosin-7B
• Protein Family: Myosin
• UniProt Gene Name: MYH7B
• UniProt Synonym Gene Names: KIAA1512

NCBI Description

The myosin II molecule is a multi-subunit complex consisting of two heavy chains and four light chains. This gene encodes a heavy chain of myosin II, which is a member of the motor-domain superfamily. The heavy chain includes a globular motor domain, which catalyzes ATP hydrolysis and interacts with actin, and a tail domain in which heptad repeat sequences promote dimerization by interacting to form a rod-like alpha-helical coiled coil. This heavy chain subunit is a slow-twitch myosin. Alternatively spliced transcript variants have been found, but the full-length nature of these variants is not determined. [provided by RefSeq, Mar 2010]

Uniprot Description

Involved in muscle contraction.