Typical Testing Data/Standard Curve (for reference only)
Human Myosin Heavy Chain 7B, Cardiac Muscle Beta Antibody (MYH7B) ELISA Kit | MYH7B elisa kit
Human Anti-Myosin Heavy Chain 7B, Cardiac Muscle Beta Antibody (Anti-MYH7B)(Competitive EIA) ELISA Kit
Gene Names
MYH7B; MHC14; MYH14
Reactivity
Human
Applications
ELISA
Synonyms
Myosin Heavy Chain 7B; Cardiac Muscle Beta Antibody (MYH7B); Human Anti-Myosin Heavy Chain 7B; Cardiac Muscle Beta Antibody (Anti-MYH7B)(Competitive EIA) ELISA Kit; ELISA Kit for Anti-Myosin Heavy Chain 7B; Cardiac Muscle Beta Antibody (Anti-MYH7B); Anti-MYH7B/Anti-Myosin Heavy Chain 7B; Cardiac Muscle Beta Antibody; MYH14; MHC14; Antigen MLAA-21; Myosin cardiac muscle beta chain; MYH7B elisa kit
Reactivity
Human
Specificity
Reactive with Human Anti-Myosin Heavy Chain 7B, Cardiac Muscle Beta Antibody (Anti-MYH7B)
This assay has high sensitivity and excellent specificity for detection of Anti-Myosin Heavy Chain 7B, Cardiac Muscle Beta Antibody (Anti-MYH7B). No significant cross-reactivity or interference between Anti-Myosin Heavy Chain 7B, Cardiac Muscle Beta Antibody (Anti-MYH7B) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Anti-Myosin Heavy Chain 7B, Cardiac Muscle Beta Antibody (Anti-MYH7B). No significant cross-reactivity or interference between Anti-Myosin Heavy Chain 7B, Cardiac Muscle Beta Antibody (Anti-MYH7B) and analogues was observed.
Applicable Applications for MYH7B elisa kit
ELISA (EIA)
Application Notes
Enzyme-linked immunosorbent assay for Antibody Detection.
Assay Type
Competitive ELISA-Antibody
Sample Types
serum, plasma and other biological fluids
Detection Method
Competitive Inhibition
Detection Range
3.12-200ng/mL
Sensitivity
1.25ng/mL
Assay Length
2.5 h
Sample Size
50ul (Please refer to the manual for the specific data.)
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Myosin Heavy Chain 7B, Cardiac Muscle Beta Antibody (Anti-MYH7B) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Myosin Heavy Chain 7B, Cardiac Muscle Beta Antibody (Anti-MYH7B) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Myosin Heavy Chain 7B, Cardiac Muscle Beta Antibody (Anti-MYH7B) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Assay Procedure Summary
1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 5 times
5. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
6. Add 50ul Stop Solution. Read at 450nm immediately.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 5 times
5. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
6. Add 50ul Stop Solution. Read at 450nm immediately.
Test Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galectin 1 (GAL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Galectin 1 (GAL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galectin 1 (GAL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Galectin 1 (GAL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Preparation and Storage
Store at 4 degree C for 1 month/ -20 degree C for long-term (within shelf life).
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Product Categories/Family for MYH7B elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,273 Da
NCBI Official Full Name
myosin-7B
NCBI Official Synonym Full Names
myosin heavy chain 7B
NCBI Official Symbol
MYH7B
NCBI Official Synonym Symbols
MHC14; MYH14
NCBI Protein Information
myosin-7B
UniProt Protein Name
Myosin-7B
Protein Family
UniProt Gene Name
MYH7B
UniProt Synonym Gene Names
KIAA1512
NCBI Description
The myosin II molecule is a multi-subunit complex consisting of two heavy chains and four light chains. This gene encodes a heavy chain of myosin II, which is a member of the motor-domain superfamily. The heavy chain includes a globular motor domain, which catalyzes ATP hydrolysis and interacts with actin, and a tail domain in which heptad repeat sequences promote dimerization by interacting to form a rod-like alpha-helical coiled coil. This heavy chain subunit is a slow-twitch myosin. Alternatively spliced transcript variants have been found, but the full-length nature of these variants is not determined. [provided by RefSeq, Mar 2010]
Uniprot Description
Involved in muscle contraction.
Research Articles on MYH7B
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Product Notes
The Human MYH7B myh7b (Catalog #AAA2709736) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2709736 ELISA Kit recognizes Human MYH7B. AAA Biotech's Myosin Heavy Chain 7B, Cardiac Muscle Beta Antibody (MYH7B) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Enzyme-linked immunosorbent assay for Antibody Detection. Researchers should empirically determine the suitability of the MYH7B myh7b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myosin Heavy Chain 7B, Cardiac Muscle Beta Antibody (MYH7B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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