Human Mucin 21 (MUC21) ELISA Kit | MUC21 elisa kit

Human Mucin 21 (MUC21) ELISA Kit

Cat. Num. AAA2704956

• Gene Names: MUC21; MUC-21; KMQK697; C6orf205
• Reactivity: Human
• Synonyms: Mucin 21 (MUC21); Human Mucin 21 (MUC21) ELISA Kit; C6orf205; Epiglycanin; MUC21 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mucin 21 (MUC21).; No significant cross-reactivity or interference between Mucin 21 (MUC21) and analogues was observed.
• Sequence Length: 416

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 21 (MUC21) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 21 (MUC21) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MUC21 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 21 (MUC21). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucin 21 (MUC21). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 21 (MUC21), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mucin 21 (MUC21) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 161016767
• NCBI GeneID: 394263
• NCBI Accession #: NP_001010909.2
• NCBI GenBank Nucleotide #: NM_001010909.4
• UniProt Accession #: Q5SSG8; Q0VGF1; Q3B7T2; Q5SS94; Q6UXC5; B0UZT7; B4DQ55; C9JMK2; D9N007
• Molecular Weight: 40,331 Da
• NCBI Official Full Name: mucin-21 isoform 1
• NCBI Official Synonym Full Names: mucin 21, cell surface associated
• NCBI Official Symbol: MUC21
• NCBI Official Synonym Symbols: MUC-21; KMQK697; C6orf205
• NCBI Protein Information: mucin-21
• UniProt Protein Name: Mucin-21
• Protein Family: Mucin
• UniProt Gene Name: MUC21
• UniProt Synonym Gene Names: C6orf205; MUC-21

NCBI Description

This gene encodes a large membrane-bound glycoprotein which is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. The encoded protein contains an N-terminal signal sequence, an extracellular mucin domain, a stem domain, a transmembrane domain, and a C-terminal cytoplasmic tail domain. The mucin domain contains O-glycosylation sites and is polymorphic with isoforms containing a variable number of nonidentical proline-, threonine-, and serine-rich tandem repeats of 15 amino acids each. The aberrent expression of this gene is associated with lung adenocarcinoma. [provided by RefSeq, May 2017]

Uniprot Description

MiscellaneousCould be considered as a marker for lung carcinomas.