Human Mitochondrial Ribosomal Protein L53 (MRPL53) ELISA Kit | MRPL53 elisa kit

Human Mitochondrial Ribosomal Protein L53 (MRPL53) ELISA Kit

Cat. Num. AAA2098136

• Gene Names: MRPL53; L53MT
• Reactivity: Human
• Synonyms: Mitochondrial Ribosomal Protein L53 (MRPL53); Human Mitochondrial Ribosomal Protein L53 (MRPL53) ELISA Kit; L53mt; 39S ribosomal protein L53; mitochondrial; MRPL53 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mitochondrial Ribosomal Protein L53 (MRPL53).; No significant cross-reactivity or interference between Mitochondrial Ribosomal Protein L53 (MRPL53) and analogues was observed.
• Sequence Length: 112

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mitochondrial Ribosomal Protein L53 (MRPL53) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mitochondrial Ribosomal Protein L53 (MRPL53) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MRPL53 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitochondrial Ribosomal Protein L53 (MRPL53). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitochondrial Ribosomal Protein L53 (MRPL53). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitochondrial Ribosomal Protein L53 (MRPL53), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mitochondrial Ribosomal Protein L53 (MRPL53) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MRPL53 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 119620057
• NCBI GeneID: 116540
• Molecular Weight: 12,107 Da
• NCBI Official Full Name: mitochondrial ribosomal protein L53
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein L53
• NCBI Official Symbol: MRPL53
• NCBI Official Synonym Symbols: L53MT
• NCBI Protein Information: 39S ribosomal protein L53, mitochondrial
• UniProt Protein Name: 39S ribosomal protein L53, mitochondrial
• Protein Family: 39S ribosomal protein
• UniProt Gene Name: MRPL53
• UniProt Synonym Gene Names: L53mt; MRP-L53

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 1p. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPL53: Component of the mitochondrial ribosome large subunit (39S) which comprises a 16S rRNA and about 50 distinct proteins. {ECO:0000269|PubMed:25278503, ECO:0000269|PubMed:25838379}

Protein type: Mitochondrial; Ribosomal

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: mitochondrial inner membrane; mitochondrial large ribosomal subunit

Molecular Function: protein binding