Human Mitochondrial Ribosomal Protein L18 (MRPL18) ELISA Kit | MRPL18 elisa kit

Human Mitochondrial Ribosomal Protein L18 (MRPL18) ELISA Kit

Cat. Num. AAA2707160

• Gene Names: MRPL18; L18mt; HSPC071; MRP-L18
• Reactivity: Human
• Synonyms: Mitochondrial Ribosomal Protein L18 (MRPL18); Human Mitochondrial Ribosomal Protein L18 (MRPL18) ELISA Kit; L18mt; 39S ribosomal protein L18; mitochondrial; MRPL18 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mitochondrial Ribosomal Protein L18 (MRPL18).; No significant cross-reactivity or interference between Mitochondrial Ribosomal Protein L18 (MRPL18) and analogues was observed.
• Sequence Length: 180

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.061ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mitochondrial Ribosomal Protein L18 (MRPL18) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mitochondrial Ribosomal Protein L18 (MRPL18) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MRPL18 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitochondrial Ribosomal Protein L18 (MRPL18). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitochondrial Ribosomal Protein L18 (MRPL18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitochondrial Ribosomal Protein L18 (MRPL18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mitochondrial Ribosomal Protein L18 (MRPL18) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 12804435
• NCBI GeneID: 29074
• Molecular Weight: 20,577 Da
• NCBI Official Full Name: Mitochondrial ribosomal protein L18
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein L18
• NCBI Official Symbol: MRPL18
• NCBI Official Synonym Symbols: L18mt; HSPC071; MRP-L18
• NCBI Protein Information: 39S ribosomal protein L18, mitochondrial
• UniProt Protein Name: 39S ribosomal protein L18, mitochondrial
• UniProt Gene Name: MRPL18
• UniProt Synonym Gene Names: L18mt; MRP-L18

NCBI Description

This nuclear gene encodes a protein component of the larger 39S subunit of mitochondrial ribosome. This protein may also aid in the import of nuclear-encoded 5S rRNA into mitochondria. Alternative splicing results in multiple transcript variants, most of which are not predicted to encode a protein. A pseudogene of this gene is found on chromosome 16. [provided by RefSeq, Jan 2016]

Uniprot Description

MRPL18: Together with thiosulfate sulfurtransferase (TST), acts as a mitochondrial import factor for the cytosolic 5S rRNA. The precursor form shows RNA chaperone activity; is able to fold the 5S rRNA into an import-competent conformation that is recognized by rhodanese (TST). Both the cytoplasmic and mitochondrial forms are able to bind to the helix IV-loop D in the gamma domain of the 5S rRNA. Belongs to the ribosomal protein L18P family.

Protein type: Mitochondrial; Ribosomal; Translation

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: extracellular space; mitochondrial inner membrane; mitochondrial large ribosomal subunit; mitochondrial ribosome; mitochondrion

Molecular Function: 5S rRNA binding; structural constituent of ribosome

Biological Process: translation