Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Mannan-binding lectin serine protease 1 (MASP1) Standard Curve.)
Mouse Mannan-Binding Lectin Serine Protease 1 (MASP1) ELISA Kit | MASP1 elisa kit
Mouse Mannan-Binding Lectin Serine Protease 1 (MASP1) ELISA Kit
Gene Names
Masp1; CCPII; Crarf; Masp1/3; AW048060
Reactivity
Mouse
Synonyms
Mannan-Binding Lectin Serine Protease 1 (MASP1); Mouse Mannan-Binding Lectin Serine Protease 1 (MASP1) ELISA Kit; MASP1; CRARF; CRARF1; DKFZp686I01199; FLJ26383; MASP; MGC126283; MGC126284; PRSS5; RaRF; Ra-reactive factor serine protease p100; manan-binding lectin serine protease-1; mannan-binding lectin serine protease; MASP1 elisa kit
Reactivity
Mouse
Specificity
Mouse Mannan-binding lectin serine protease 1 (MASP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MASP1. No significant cross-reactivity or interference between Mouse MASP1 and analogues was observed.
Sequence Length
704
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Mannan-binding lectin serine protease 1 (MASP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Mannan-binding lectin serine protease 1 (MASP1) Standard Curve.)
Related Product Information for MASP1 elisa kit
Description: This Mouse Mannan-binding lectin serine protease 1 (MASP1) ELISA Kit employs a two-site sandwich ELISA to quantitate MASP1 in samples. An antibody specific for MASP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MASP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MASP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MASP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Mannose-associated serine protease 1 (MASP1) is involved in the lectin pathway of the complement system and is responsible for cleaving C4 and C2 to form C4b2a, a C3-convertase.Mannose-binding lectin (MBL) is a serum component which participates in innate immunity by activating complement via a novel pathway. Human MBL forms complexes with two types of serine proteases termed MASP (MBL-associated serine protease). These two proteases, MASP1 and MASP2, are structurally similar to one another as well as to C1r and C1s. Together, MASP, C1r and C1s constitute a novel serine protease family. It is likely that human MASP1 is able to activate C3, while human MASP2 cleaves C4, although further functional studies are required to confirm this.
Background: Mannose-associated serine protease 1 (MASP1) is involved in the lectin pathway of the complement system and is responsible for cleaving C4 and C2 to form C4b2a, a C3-convertase.Mannose-binding lectin (MBL) is a serum component which participates in innate immunity by activating complement via a novel pathway. Human MBL forms complexes with two types of serine proteases termed MASP (MBL-associated serine protease). These two proteases, MASP1 and MASP2, are structurally similar to one another as well as to C1r and C1s. Together, MASP, C1r and C1s constitute a novel serine protease family. It is likely that human MASP1 is able to activate C3, while human MASP2 cleaves C4, although further functional studies are required to confirm this.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mannan-binding lectin serine protease 1 isoform 2
NCBI Official Synonym Full Names
mannan-binding lectin serine peptidase 1
NCBI Official Symbol
Masp1
NCBI Official Synonym Symbols
CCPII; Crarf; Masp1/3; AW048060
NCBI Protein Information
mannan-binding lectin serine protease 1
UniProt Protein Name
Mannan-binding lectin serine protease 1
Protein Family
UniProt Gene Name
Masp1
UniProt Synonym Gene Names
Crarf; Masp3; MASP-1; RaRF
Uniprot Description
Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5.
Research Articles on MASP1
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Product Notes
The Mouse MASP1 masp1 (Catalog #AAA9717730) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717730 ELISA Kit recognizes Mouse MASP1. It is sometimes possible for the material contained within the vial of "Mannan-Binding Lectin Serine Protease 1 (MASP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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