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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Mesoderm-specific transcript homolog protein (MEST) Standard Curve.)

Human Mesoderm-Specific Transcript Homolog Protein (MEST) ELISA Kit | MEST elisa kit

Human Mesoderm-Specific Transcript Homolog Protein (MEST) ELISA Kit

Gene Names
MEST; PEG1
Reactivity
Human
Synonyms
Mesoderm-Specific Transcript Homolog Protein (MEST); Human Mesoderm-Specific Transcript Homolog Protein (MEST) ELISA Kit; MEST; DKFZp686L18234; MGC111102; MGC8703; PEG1; mesoderm specific transcript; paternally expressed gene 1; MEST elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Human Mesoderm-specific transcript homolog protein (MEST) ELISA Kit has high sensitivity and excellent specificity for detection of Human MEST. No significant cross-reactivity or interference between Human MEST and analogues was observed.
Sequence Length
335
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Mesoderm-specific transcript homolog protein (MEST) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Mesoderm-specific transcript homolog protein (MEST) Standard Curve.)
Related Product Information for MEST elisa kit
Description: This Human Mesoderm-specific transcript homolog protein (MEST) ELISA Kit employs a two-site sandwich ELISA to quantitate MEST in samples. An antibody specific for MEST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MEST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MEST is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MEST bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: MEST encodes a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. Mesoderm-specific transcript homolog protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for MEST. Pseudogenes of MEST are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Mesoderm-specific transcript homolog protein
NCBI Official Synonym Full Names
mesoderm specific transcript
NCBI Official Symbol
MEST
NCBI Official Synonym Symbols
PEG1
NCBI Protein Information
mesoderm-specific transcript homolog protein
UniProt Protein Name
Mesoderm-specific transcript homolog protein
UniProt Gene Name
MEST
UniProt Synonym Gene Names
PEG1
UniProt Entry Name
MEST_HUMAN

NCBI Description

This gene encodes a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15. [provided by RefSeq, Dec 2011]

Uniprot Description

MEST: a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15. [provided by RefSeq, Dec 2011]

Protein type: Membrane protein, multi-pass; Membrane protein, integral; EC 3.-.-.-

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: hydrolase activity

Biological Process: response to retinoic acid; metabolic process; mesoderm development

Research Articles on MEST

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Product Notes

The Human MEST mest (Catalog #AAA9715309) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715309 ELISA Kit recognizes Human MEST. It is sometimes possible for the material contained within the vial of "Mesoderm-Specific Transcript Homolog Protein (MEST), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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