Human PLAT/TPA ELISA Kit | PLAT elisa kit

Human PLAT/TPA PicoKine ELISA Kit

Cat. Num. AAA177399

• Gene Names: PLAT; TPA; T-PA
• Reactivity: Human
• Synonyms: PLAT/TPA; Human PLAT/TPA PicoKine ELISA Kit; Tissue-type plasminogen activator; plasminogen activator; tissue; Alteplase; Plasminogen activator tissue; Plasminogen activator tissue type; PLAT; Reteplase; t-PA; T-plasminogen activator; Tissue-type plasminogen activator chain B; tPA; TPA_HUMAN; PLAT elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human PLAT
• Sequence Length: 562

• Samples: Cell culture supernates, serum, plasma (heparin, EDTA, citrate) and urine
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human PLAT/TPA PicoKine ELISA Kit standard curve )

Related Product Information for PLAT elisa kit

Principle of the assay: human PLAT ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PLAT has been precoated onto 96-well plates. Standards(CHO, M1-P562) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLAT is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PLAT amount of sample captured in plate.
Background: Plasminogen activator, tissue, also called PLAT or TPA is a serine protease. This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This gene was mapped to 8p11.21. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism.

NCBI and Uniprot Product Information

• NCBI GI #: 137119
• NCBI GeneID: 5327
• UniProt Accession #: P00750; Q15103; Q503B0; Q6PJA5; Q7Z7N2; Q86YK8; Q9BU99; Q9BZW1; A8K022; B2R8E8
• Molecular Weight: 44,373 Da
• NCBI Official Full Name: Tissue-type plasminogen activator
• NCBI Official Synonym Full Names: plasminogen activator, tissue
• NCBI Official Symbol: PLAT
• NCBI Official Synonym Symbols: TPA; T-PA
• NCBI Protein Information: tissue-type plasminogen activator; alteplase; reteplase; t-plasminogen activator; plasminogen activator, tissue type; tissue plasminogen activator (t-PA)
• UniProt Protein Name: Tissue-type plasminogen activator
• Protein Family: Tissue-type plasminogen activator
• UniProt Gene Name: PLAT
• UniProt Synonym Gene Names: t-PA; t-plasminogen activator; tPA
• UniProt Entry Name: TPA_HUMAN

NCBI Description

This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PLAT: Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration. Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. Belongs to the peptidase S1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; EC 3.4.21.68; Vesicle; Protease

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: extracellular matrix; extracellular space; cell surface; apical part of cell; cytoplasm; extracellular region; synapse; secretory granule

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: negative regulation of proteolysis; response to peptide hormone stimulus; synaptic transmission, glutamatergic; fibrinolysis; response to cAMP; smooth muscle cell migration; response to hypoxia; protein modification process; regulation of synaptic plasticity; platelet-derived growth factor receptor signaling pathway; proteolysis; blood coagulation; transmembrane transport; plasminogen activation

Disease: Thrombophilia, Familial, Due To Decreased Release Of Tissue Plasminogen Activator