Rabbit anti-Human MEF2C Polyclonal Antibody | anti-MEF2C antibody

MEF2C (T300) (Myocyte-specific Enhancer Factor 2C) (PE)

Cat. Num. AAA6489004

• Gene Names: MEF2C; DEL5q14.3; C5DELq14.3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: MEF2C; Polyclonal Antibody; MEF2C (T300) (Myocyte-specific Enhancer Factor 2C) (PE); anti-MEF2C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MEF2C.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MEF2C antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acid residues surrounding T300 of human MEF2C (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MEF2C (arrow) using rabbit polyclonal MEF2C Antibody (T300) (RB11009). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MEF2C gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with MEF2C Antibody (T300), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-MEF2C antibody

Antibodies; Transcription Factors

References

1. Konig, S., et al., J. Biol. Chem. 279(27):28187-28196 (2004). 2. Maeda, T., et al., J. Biol. Chem. 277(50):48889-48898 (2002). 3. Maeda, T., et al., Biochem. Biophys. Res. Commun. 294(4):791-797 (2002). 4. Janson, C.G., et al., Brain Res. Mol. Brain Res. 97(1):70-82 (2001). 5. Krainc, D., et al., Genomics 29(3):809-811 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 19923215
• NCBI GeneID: 4208
• NCBI Accession #: NP_002388
• NCBI GenBank Nucleotide #: NM_002397
• UniProt Accession #: Q06413; C9JMZ0; D7F7N5; F8W7V7
• Molecular Weight: 50,242 Da
• NCBI Official Full Name: myocyte-specific enhancer factor 2C isoform 1
• NCBI Official Synonym Full Names: myocyte enhancer factor 2C
• NCBI Official Symbol: MEF2C
• NCBI Official Synonym Symbols: DEL5q14.3; C5DELq14.3
• NCBI Protein Information: myocyte-specific enhancer factor 2C; MADS box transcription enhancer factor 2, polypeptide C
• UniProt Protein Name: Myocyte-specific enhancer factor 2C
• Protein Family: Myocyte-specific enhancer factor
• UniProt Gene Name: MEF2C
• UniProt Entry Name: MEF2C_HUMAN

Uniprot Description

MEF2C: transcription factor of the MADS family which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. May be involved in myogenesis, neurogenesis and in the development of cortical architecture. Three splice-variant isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 5q14.3

Cellular Component: nucleoplasm; protein complex; intracellular membrane-bound organelle; cytoplasm; nuclear speck; nucleus; cytosol

Molecular Function: protein binding; miRNA binding; transcription activator binding; DNA binding; AT DNA binding; protein heterodimerization activity; histone deacetylase binding; chromatin binding; transcription factor activity

Biological Process: cardiac muscle hypertrophy; muscle development; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; heart development; regulation of neurotransmitter secretion; stress-activated MAPK cascade; regulation of synaptic plasticity; toll-like receptor 3 signaling pathway; toll-like receptor 5 signaling pathway; neuron differentiation; positive regulation of MAP kinase activity; B cell receptor signaling pathway; regulation of neuron apoptosis; negative regulation of ossification; positive regulation of B cell proliferation; chondrocyte differentiation; negative regulation of neuron apoptosis; toll-like receptor 4 signaling pathway; positive regulation of cardiac muscle cell proliferation; endochondral ossification; nervous system development; skeletal muscle development; monocyte differentiation; response to virus; myotube differentiation; positive regulation of skeletal muscle development; muscle cell fate determination; toll-like receptor 2 signaling pathway; muscle cell differentiation; regulation of synaptogenesis; neuron development; positive regulation of transcription from RNA polymerase II promoter; smooth muscle cell differentiation; toll-like receptor 9 signaling pathway; B cell proliferation; transcription from RNA polymerase II promoter; regulation of synaptic transmission, glutamatergic; apoptosis; neuron migration; ventricular cardiac muscle cell differentiation; regulation of synaptic activity; negative regulation of transcription from RNA polymerase II promoter; palate development; toll-like receptor 10 signaling pathway; learning and/or memory; B cell homeostasis; regulation of transcription, DNA-dependent; melanocyte differentiation; regulation of megakaryocyte differentiation; germinal center formation; neural crest cell differentiation; heart looping; negative regulation of epithelial cell proliferation; blood vessel development; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; embryonic viscerocranium morphogenesis; positive regulation of bone mineralization; humoral immune response; regulation of germinal center formation; positive regulation of myoblast differentiation; osteoblast differentiation; MyD88-dependent toll-like receptor signaling pathway; positive regulation of osteoblast differentiation; neuron morphogenesis during differentiation; toll-like receptor signaling pathway; innate immune response; positive regulation of muscle cell differentiation; blood vessel remodeling; platelet formation; regulation of excitatory postsynaptic membrane potential; positive regulation of neuron differentiation

Disease: Mental Retardation, Autosomal Dominant 20