Human major histocompatibility complex class I chain-related gene B, MICB ELISA Kit | MICB elisa kit

Human major histocompatibility complex class I chain-related gene B, MICB ELISA Kit

Cat. Num. AAA161121

• Gene Names: MICB; PERB11.2
• Reactivity: Human
• Synonyms: major histocompatibility complex class I chain-related gene B; MICB; Human major histocompatibility complex class I chain-related gene B; MICB ELISA Kit; MICB elisa kit

• Reactivity: Human
• Sequence Length: 317

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.2ng/ml - 60ng/ml
• Sensitivity: 0.117ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MICB elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Major Histocompatibility Complex Class I Chain-related Gene B (also known as MICB) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human MICB antibody. MICB present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human MICB Antibody is added and binds to MICB in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MICB antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human MICB. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 302310538
• NCBI GeneID: 4277
• Molecular Weight: 37,651 Da
• NCBI Official Full Name: MHC class I chain-related protein B, partial
• NCBI Official Synonym Full Names: MHC class I polypeptide-related sequence B
• NCBI Official Symbol: MICB
• NCBI Official Synonym Symbols: PERB11.2
• NCBI Protein Information: MHC class I polypeptide-related sequence B
• UniProt Protein Name: MHC class I polypeptide-related sequence B
• Protein Family: MHC class I polypeptide-related sequence
• UniProt Gene Name: MICB
• UniProt Synonym Gene Names: MIC-B

NCBI Description

This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.