Human LOXL2 PicoKine ELISA Kit | LOXL2 elisa kit

Human LOXL2 PicoKine ELISA Kit

Cat. Num. AAA177562

• Gene Names: LOXL2; LOR2; WS9-14
• Reactivity: Human
• Synonyms: LOXL2 PicoKine; Human LOXL2 PicoKine ELISA Kit; Lysyl oxidase homolog 2; LOR 2; LOR2; LOX L2; LOXL 2; LOXL2; LOXL2_HUMAN; Lysyl oxidase like 2; Lysyl oxidase like protein 2; Lysyl oxidase related 2; Lysyl oxidase related protein 2; Lysyl oxidase related protein WS9 14; Lysyl oxidase-like protein 2; Lysyl oxidase-related protein 2; Lysyl oxidase-related protein WS9-14; WS9 14 ; lysyl oxidase-like 2; LOXL2 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human LOXL2
• Sequence Length: 774

• Assay Range: 156pg/ml-10,000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of human LOXL2 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma(heparin, EDTA).
• Immunogen: NSO, Q26-Q774
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 10,000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human LOXL2 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human LOXL2 standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|1589|3122|7510|1466|4236|6998; Standard deviation|79.45|171.71|435.58|76.23|258.39|447.87; CV(%)|5.0|5.5|5.8|5.2|6.1|6.4
• Sample Data: Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10,000; O.D.|0.046|0.178|0.288|0.519|0.820|1.335|1.889|2.426
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human LOXL2 PicoKine ELISA Kit standard curve )

Related Product Information for LOXL2 elisa kit

Principle of the assay: Human LOXL2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LOXL2 has been precoated onto 96-well plates. Standards (NSO,Q26-Q774) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LOXL2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human LOXL2 amount of sample captured in plate.

Background: Lysyl oxidase homolog 2 is an enzyme that in humans is encoded by the LOXL2 gene. This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. LOXL2 can also crosslink collagen type IV and hence influence the sprouting of new blood vessels.

NCBI and Uniprot Product Information

• NCBI GI #: 4505011
• NCBI GeneID: 4017
• NCBI Accession #: NP_002309.1
• NCBI GenBank Nucleotide #: NM_002318.2
• UniProt Accession #: Q9Y4K0; Q53HV3; Q9BW70; Q9Y5Y8; B2R5Q0
• Molecular Weight: 86,725 Da
• NCBI Official Full Name: lysyl oxidase homolog 2
• NCBI Official Synonym Full Names: lysyl oxidase like 2
• NCBI Official Symbol: LOXL2
• NCBI Official Synonym Symbols: LOR2; WS9-14
• NCBI Protein Information: lysyl oxidase homolog 2
• UniProt Protein Name: Lysyl oxidase homolog 2
• Protein Family: Lysyl oxidase
• UniProt Gene Name: LOXL2
• UniProt Entry Name: LOXL2_HUMAN

NCBI Description

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq, Jul 2008]

Uniprot Description

LOXL2: Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When nuclear, acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E- cadherin, probably by mediating deamination of histone H3. Also involved in E-cadherin repression following hypoxia, a hallmark of epithelial to mesenchymal transition believed to amplify tumor aggressiveness, suggesting that it may play a role in tumor progression. Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation. Belongs to the lysyl oxidase family.

Protein type: Secreted, signal peptide; Secreted; Cell adhesion; Oxidoreductase; EC 1.4.3.13

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: basement membrane; chromosome; extracellular space; membrane; nucleoplasm; nucleus

Molecular Function: chromatin binding; copper ion binding; electron carrier activity; methylated histone residue binding; protein binding; protein-lysine 6-oxidase activity; scavenger receptor activity; transcription corepressor activity

Biological Process: aging; cell adhesion; collagen fibril organization; endothelial cell migration; endothelial cell proliferation; epithelial to mesenchymal transition; histone modification; negative regulation of transcription, DNA-dependent; positive regulation of chondrocyte differentiation; protein amino acid deamination; protein modification process; receptor-mediated endocytosis; response to copper ion; response to hypoxia; sprouting angiogenesis; transcription, DNA-dependent