Human Lysyl oxidase homolog 2 ELISA Kit | LOXL2 elisa kit

Human Lysyl oxidase homolog 2 ELISA Kit

Cat. Num. AAA761786

• Gene Names: LOXL2; LOR2; WS9-14
• Reactivity: Human
• Synonyms: Lysyl oxidase homolog 2; Human Lysyl oxidase homolog 2 ELISA Kit; LOXL2/Lysyl Oxidase Homolog 2/LOL2/LOR2/lysyl oxidase related 2/lysyl oxidase-like 2/Lysyl oxidase-like protein 2/Lysyl oxidase-related protein 2/Lysyl oxidase-related protein WS9-14/WS9-14; LOXL2 elisa kit

• Reactivity: Human
• Sequence Length: 774

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 37.5-2400pg/ml
• Sensitivity: 22.5pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for LOXL2 elisa kit

Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LOXL2. During the reaction,Human LOXL2 in the sample or standard competes with a fixed amount of biotin-labeled Human LOXL2 for sites on a pre-coated Monoclonal antibody specific to Human LOXL2. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human LOXL2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4505011
• NCBI GeneID: 4017
• NCBI Accession #: NP_002309.1
• NCBI GenBank Nucleotide #: NM_002318.2
• Molecular Weight: 86,725 Da
• NCBI Official Full Name: lysyl oxidase homolog 2
• NCBI Official Synonym Full Names: lysyl oxidase like 2
• NCBI Official Symbol: LOXL2
• NCBI Official Synonym Symbols: LOR2; WS9-14
• NCBI Protein Information: lysyl oxidase homolog 2
• UniProt Protein Name: Lysyl oxidase homolog 2
• Protein Family: Lysyl oxidase
• UniProt Gene Name: LOXL2
• UniProt Entry Name: LOXL2_HUMAN

NCBI Description

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq, Jul 2008]

Uniprot Description

LOXL2: Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When nuclear, acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E- cadherin, probably by mediating deamination of histone H3. Also involved in E-cadherin repression following hypoxia, a hallmark of epithelial to mesenchymal transition believed to amplify tumor aggressiveness, suggesting that it may play a role in tumor progression. Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation. Belongs to the lysyl oxidase family.

Protein type: Cell adhesion; EC 1.4.3.13; Oxidoreductase; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: basement membrane; chromosome; extracellular space; membrane; nucleoplasm; nucleus

Molecular Function: chromatin binding; copper ion binding; electron carrier activity; methylated histone residue binding; protein binding; protein-lysine 6-oxidase activity; scavenger receptor activity; transcription corepressor activity

Biological Process: aging; cell adhesion; collagen fibril organization; endothelial cell migration; endothelial cell proliferation; epithelial to mesenchymal transition; histone modification; negative regulation of transcription, DNA-dependent; positive regulation of chondrocyte differentiation; protein amino acid deamination; protein modification process; receptor-mediated endocytosis; response to copper ion; response to hypoxia; sprouting angiogenesis; transcription, DNA-dependent