Typical Testing Data/Standard Curve (for reference only)
Human Leptin ELISA Kit | LEP elisa kit
Human Leptin ELISA Kit
Gene Names
LEP; OB; OBS; LEPD
Reactivity
Human
Applications
ELISA
Synonyms
Leptin; Human Leptin ELISA Kit; LEP elisa kit
Reactivity
Human
Specificity
The following substances were tested and found to have no cross-reactivity: human IL-1alpha, human IL-1beta, human IL-2, human IL-3, human IL-4, human IL-5, human IL-6, human IL-7, human IL-8, human IL-15? human IFN?-gamma? human TNF?-alpha?
Sequence Length
167
Applicable Applications for LEP elisa kit
ELISA (EIA)
Samples
Biological samples of human
Assay Type
Sandwich
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LEP elisa kit
Intended Uses: The Human Leptin (LPT) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring leptin in biological samples of human.
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the LPT present in samples reacts with the anti-LPT antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-LPT, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of LPT in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of LPT in the test sample. The quantity of LPT in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the LPT present in samples reacts with the anti-LPT antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-LPT, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of LPT in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of LPT in the test sample. The quantity of LPT in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
leptin
NCBI Official Synonym Full Names
leptin
NCBI Official Symbol
LEP
NCBI Official Synonym Symbols
OB; OBS; LEPD
NCBI Protein Information
leptin
Protein Family
NCBI Description
This gene encodes a protein that is secreted by white adipocytes into the circulation and plays a major role in the regulation of energy homeostasis. Circulating leptin binds to the leptin receptor in the brain, which activates downstream signaling pathways that inhibit feeding and promote energy expenditure. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation and wound healing. Mutations in this gene and its regulatory regions cause severe obesity and morbid obesity with hypogonadism in human patients. A mutation in this gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Aug 2017]
Research Articles on LEP
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Product Notes
The Human LEP (Catalog #AAA560910) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA560910 ELISA Kit recognizes Human LEP. AAA Biotech's Leptin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the LEP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Leptin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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