Rabbit anti-Mouse Npr1 Polyclonal Antibody | anti-Npr1 antibody

Npr1, NT (Npr1, Npra, Atrial natriuretic peptide receptor 1, Atrial natriuretic peptide receptor type A, Guanylate cyclase A) (PE)

Cat. Num. AAA6326324

• Reactivity: Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Npr1; Polyclonal Antibody; NT (Npr1; Npra; Atrial natriuretic peptide receptor 1; Atrial natriuretic peptide receptor type A; Guanylate cyclase A) (PE); Guanylate cyclase A; anti-Npr1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-Npr1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Mouse Npr1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 197-226 amino acids from the N-terminal region of human Mouse Npr1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Npr1 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Npr1 antibody detected the Npr1 protein (arrow).)

Immunohistochemistry (IHC)

(Mouse Npr1 Antibody (N-term) (AP14073a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse duodenum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Npr1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-Npr1 antibody

Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.

Product Categories/Family for anti-Npr1 antibody

Antibodies; Receptors

References

Madhani, M., et al. Am. J. Physiol. Heart Circ. Physiol. 299 (3), H827-H836 (2010): Panayiotou, C.M., et al. Br. J. Pharmacol. 160(8):2045-2054(2010). Ropero, A.B., et al. Endocrinology 151(8):3665-3674(2010). Tankersley, C.G., et al. Inhal Toxicol 22(8):695-707(2010). Long, L., et al. Neuro Endocrinol. Lett. 31(1):126-130(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 113930718
• NCBI GeneID: 18160
• NCBI Accession #: NP_032753.5
• NCBI GenBank Nucleotide #: NM_008727.5
• UniProt Accession #: P18293
• Molecular Weight: 119,109 Da
• NCBI Official Full Name: atrial natriuretic peptide receptor 1
• UniProt Protein Name: Atrial natriuretic peptide receptor 1
• Protein Family: Nitrogen permease reactivator protein
• UniProt Gene Name: Npr1
• UniProt Synonym Gene Names: Npra; ANP-A; ANPR-A; NPR-A; GC-A

Uniprot Description

ANPA: natriuretic peptide receptor A, which mediates natriuretic, diuretic, and vasorelaxing actions of the natriuretic peptides. Contains five functional domains: an extracellular ligand-binding domain, a single membrane-spanning region, and intracellularly a protein kinase homology domain, a helical hinge region involved in oligomerization, and a carboxyl-terminal guanylyl cyclase catalytic domain. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Has guanylate cyclase activity on binding of ANF. Hypertensive SHR Rat has polymorphism at the locus. Human non-coding polymorphisms can alter expression up to two-fold, and have been associated with hypertension. A coding SNP is associated with hypertension and myocardial infarction.

Protein type: EC 4.6.1.2; Guanylyl cyclase; Kinase, protein; Lyase; Membrane protein, integral; Nucleotide Metabolism - purine; Protein kinase, RGC; Protein kinase, dual-specificity (receptor); RGC family; RGC group; Receptor, misc.

Chromosomal Location of Human Ortholog: 3 F1|3 39.23 cM

Cellular Component: guanylate cyclase complex, soluble; plasma membrane; receptor complex

Molecular Function: GTP binding; guanylate cyclase activity; hormone binding; natriuretic peptide receptor activity; peptide hormone binding; protein kinase binding

Biological Process: cell surface receptor linked signal transduction; cGMP biosynthetic process; dopamine metabolic process; negative regulation of smooth muscle cell proliferation; positive regulation of cGMP biosynthetic process; receptor guanylyl cyclase signaling pathway; regulation of blood pressure