Sheep STAT6 ELISA Kit | STAT56 elisa kit

Sheep STAT6 (Signal Transducer and Activator of Transcription 6) ELISA Kit

Cat. Num. AAA2508366

• Gene Names: STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT
• Reactivity: Sheep
• Synonyms: STAT6; Sheep STAT6 (Signal Transducer and Activator of Transcription 6) ELISA Kit; STAT56 elisa kit

• Reactivity: Sheep
• Specificity: This kit recognizes natural and recombinant Sheep STAT6. No significant cross-reactivity or interference between Sheep STAT6 and analogues was observed.
• Sequence Length: 847

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 7.813-500pg/mL
• Sensitivity: Min: 4.688pg/mL; Max: 500pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for STAT56 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep STAT6 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to STAT6. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for STAT6 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain STAT6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of STAT6. You can calculate the concentration of STAT6 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 296010864
• NCBI GeneID: 6778
• NCBI Accession #: NP_001171549.1
• NCBI GenBank Nucleotide #: NM_001178078.1
• UniProt Accession #: P42226; Q5FBW5; Q71UP4; A8K316; B7ZA27; F5GXI9
• Molecular Weight: 81,748 Da
• NCBI Official Full Name: signal transducer and activator of transcription 6 isoform 1
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 6, interleukin-4 induced
• NCBI Official Symbol: STAT6
• NCBI Official Synonym Symbols: STAT6B; STAT6C; D12S1644; IL-4-STAT
• NCBI Protein Information: signal transducer and activator of transcription 6; STAT, interleukin4-induced; transcription factor IL-4 STAT
• UniProt Protein Name: Signal transducer and activator of transcription 6
• UniProt Gene Name: STAT6
• UniProt Entry Name: STAT6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

STAT6: transcription factor of the STAT family. Plays a central role in IL4-mediated biological responses. Induces the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. May function in the differentiation of T helper 2 cells and class switch of immunoglobulins. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; nuclear chromatin; cytoplasm; cytosol; lipid raft

Molecular Function: identical protein binding; signal transducer activity; protein binding; transcription factor activity; protein phosphatase binding

Biological Process: regulation of transcription from RNA polymerase II promoter; T-helper 1 cell lineage commitment; transcription, DNA-dependent; negative regulation of T-helper 2 type immune response; positive regulation of interferon type I production; mammary gland epithelial cell proliferation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of isotype switching to IgE isotypes; regulation of cell proliferation