Human Kruppel Like Factor 4, Gut (KLF4) ELISA Kit | KLF4 elisa kit

Human Kruppel Like Factor 4, Gut (KLF4) ELISA Kit

Cat. Num. AAA2019956

• Gene Names: KLF4; EZF; GKLF
• Reactivity: Human
• Synonyms: Kruppel Like Factor 4; Gut (KLF4); Human Kruppel Like Factor 4; Gut (KLF4) ELISA Kit; EZF; GKLF; Gut-Enriched Krüppel-Like Factor; Epithelial zinc finger protein EZF; KLF4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of KLF4. No significant cross-reactivity or interference between KLF4 and analogues was observed.
• Sequence Length: 479

• Samples: Human tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level KLF4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level KLF4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KLF4 elisa kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to KLF4. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to KLF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain KLF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of KLF4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for KLF4 elisa kit

Signal transduction; Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 194248077
• NCBI GeneID: 9314
• NCBI Accession #: NP_004226.3
• NCBI GenBank Nucleotide #: NM_004235.4
• UniProt Accession #: O43474; P78338; Q5T3J8; Q5T3J9; Q8N717; Q9UNP3; B2R8S4; B3KT79; L0R3I6; L0R4N5
• Molecular Weight: 6,489 Da
• NCBI Official Full Name: Krueppel-like factor 4
• NCBI Official Synonym Full Names: Kruppel-like factor 4 (gut)
• NCBI Official Symbol: KLF4
• NCBI Official Synonym Symbols: EZF; GKLF
• NCBI Protein Information: Krueppel-like factor 4
• UniProt Protein Name: Krueppel-like factor 4
• Protein Family: Krueppel-like factor
• UniProt Gene Name: KLF4
• UniProt Synonym Gene Names: EZF; GKLF
• UniProt Entry Name: KLF4_HUMAN

Uniprot Description

KLF4: Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. Interacts with POU5F1/OCT4 and SOX2. Interacts with MUC1 (via the C-terminal domain). Belongs to the krueppel C2H2-type zinc-finger protein family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 9q31

Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin

Molecular Function: protein binding; zinc ion binding; double-stranded DNA binding; phosphoinositide 3-kinase regulator activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; fat cell differentiation; post-embryonic camera-type eye development; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; negative regulation of interleukin-8 biosynthetic process; negative regulation of muscle hyperplasia; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; positive regulation of protein metabolic process; positive regulation of hemoglobin biosynthetic process; regulation of phosphoinositide 3-kinase activity; negative regulation of cell proliferation; post-embryonic hemopoiesis; epidermis morphogenesis; regulation of cell differentiation; response to retinoic acid; negative regulation of protein kinase B signaling cascade; negative regulation of heterotypic cell-cell adhesion; stem cell maintenance; positive regulation of telomerase activity; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; epidermal cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; mesodermal cell fate determination; negative regulation of phosphoinositide 3-kinase cascade