Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Keratin, type II cytoskeletal 1 (KRT1) Standard Curve.)
Human Keratin, Type II Cytoskeletal 1 (KRT1) ELISA Kit | KRT1 elisa kit
Human Keratin, Type II Cytoskeletal 1 (KRT1) ELISA Kit
Gene Names
KRT1; K1; CK1; EHK; EHK1; EPPK; KRT1A; NEPPK
Reactivity
Human
Synonyms
Keratin; Type II Cytoskeletal 1 (KRT1); Human Keratin; Type II Cytoskeletal 1 (KRT1) ELISA Kit; KRT1; CK1; EHK; EHK1; EPPK; K1; KRT1A; NEPPK; cytokeratin 1; epidermolytic hyperkeratosis 1; hair alpha protein; keratin; type cytoskeletal 1; KRT1 elisa kit
Reactivity
Human
Specificity
Human Keratin, type II cytoskeletal 1 (KRT1) ELISA Kit has high sensitivity and excellent specificity for detection of Human KRT1. No significant cross-reactivity or interference between Human KRT1 and analogues was observed.
Sequence Length
644
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Keratin, type II cytoskeletal 1 (KRT1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Keratin, type II cytoskeletal 1 (KRT1) Standard Curve.)
Related Product Information for KRT1 elisa kit
Description: This Human Keratin, type II cytoskeletal 1 (KRT1) ELISA Kit employs a two-site sandwich ELISA to quantitate KRT1 in samples. An antibody specific for KRT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KRT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.Keratin 1 is specifically expressed in the spinous and granular layers of the epidermis with family member keratin 10. Mutations in this gene have been associated with the variants of bullous congenital ichthyosiform erythroderma in which the palms and soles of the feet are affected.
Background: The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.Keratin 1 is specifically expressed in the spinous and granular layers of the epidermis with family member keratin 10. Mutations in this gene have been associated with the variants of bullous congenital ichthyosiform erythroderma in which the palms and soles of the feet are affected.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
keratin, type II cytoskeletal 1
NCBI Official Synonym Full Names
keratin 1
NCBI Official Symbol
KRT1
NCBI Official Synonym Symbols
K1; CK1; EHK; EHK1; EPPK; KRT1A; NEPPK
NCBI Protein Information
keratin, type II cytoskeletal 1
UniProt Protein Name
Keratin, type II cytoskeletal 1
Protein Family
UniProt Gene Name
KRT1
UniProt Synonym Gene Names
KRTA; CK-1; K1
NCBI Description
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
Uniprot Description
May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.
Research Articles on KRT1
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Product Notes
The Human KRT1 krt1 (Catalog #AAA9715552) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715552 ELISA Kit recognizes Human KRT1. It is sometimes possible for the material contained within the vial of "Keratin, Type II Cytoskeletal 1 (KRT1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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