Rabbit anti-Human KRT1 Polyclonal Antibody | anti-KRT1 antibody

KRT1, ID (KRT1, KRTA, Keratin, type II cytoskeletal 1, 67kD cytokeratin, Cytokeratin-1, Hair alpha protein, Keratin-1, Type-II keratin Kb1)

Cat. Num. AAA6002798

• Gene Names: KRT1; K1; CK1; EHK; EHK1; EPPK; KRT1A; NEPPK
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: KRT1; Polyclonal Antibody; ID (KRT1; KRTA; Keratin; type II cytoskeletal 1; 67kD cytokeratin; Cytokeratin-1; Hair alpha protein; Keratin-1; Type-II keratin Kb1); Anti -KRT1; anti-KRT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-KRT1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry; Dilution: ELISA: 1:1,000; Western Blot: 1:50-100; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: KRT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 422~451 amino acids from the Central region of human KRT1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of KRT1 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). KRT1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(KRT1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KRT1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(KRT1 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-KRT1 antibody

KRT1 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.

Product Categories/Family for anti-KRT1 antibody

Antibodies; Abs to Cytokeratin

NCBI and Uniprot Product Information

• NCBI GI #: 119395750
• NCBI GeneID: 3848
• NCBI Accession #: NP_006112.3
• NCBI GenBank Nucleotide #: NM_006121.3
• UniProt Accession #: P04264; Q14720; Q6GSJ0; Q9H298; B2RA01
• Molecular Weight: 66,039 Da
• NCBI Official Full Name: keratin, type II cytoskeletal 1
• NCBI Official Synonym Full Names: keratin 1
• NCBI Official Symbol: KRT1
• NCBI Official Synonym Symbols: K1; CK1; EHK; EHK1; EPPK; KRT1A; NEPPK
• NCBI Protein Information: keratin, type II cytoskeletal 1; CK-1; cytokeratin 1; cytokeratin-1; 67 kDa cytokeratin; hair alpha protein; type-II keratin Kb1; epidermolytic hyperkeratosis 1
• UniProt Protein Name: Keratin, type II cytoskeletal 1
• Protein Family: Keratin
• UniProt Gene Name: KRT1
• UniProt Synonym Gene Names: KRTA; CK-1; K1
• UniProt Entry Name: K2C1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]

Uniprot Description

K1: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K10.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: extracellular matrix; extracellular space; cytoskeleton; membrane; keratin filament; plasma membrane; nucleus

Molecular Function: protein binding; receptor activity; carbohydrate binding; structural molecule activity

Biological Process: retinal homeostasis; fibrinolysis; negative regulation of inflammatory response; response to oxidative stress; complement activation, lectin pathway; regulation of angiogenesis

Disease: Palmoplantar Keratoderma, Epidermolytic; Keratosis Palmoplantaris Striata Iii; Ichthyosis Hystrix, Curth-macklin Type; Palmoplantar Keratoderma, Nonepidermolytic; Epidermolytic Hyperkeratosis; Ichthyosis, Cyclic, With Epidermolytic Hyperkeratosis