Human Keratin, Type I Cytoskeletal 12 (KRT12) ELISA Kit | KRT12 elisa kit

Human Keratin, Type I Cytoskeletal 12 (KRT12) ELISA Kit

Cat. Num. AAA9715550

• Gene Names: KRT12; K12
• Reactivity: Human
• Synonyms: Keratin; Type I Cytoskeletal 12 (KRT12); Human Keratin; Type I Cytoskeletal 12 (KRT12) ELISA Kit; KRT12; K12; KRT12 elisa kit

• Reactivity: Human
• Specificity: Human Keratin, type I cytoskeletal 12 (KRT12) ELISA Kit has high sensitivity and excellent specificity for detection of Human KRT12. No significant cross-reactivity or interference between Human KRT12 and analogues was observed.
• Sequence Length: 494

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Keratin, type I cytoskeletal 12 (KRT12) Standard Curve.)

Related Product Information for KRT12 elisa kit

Description: This Human Keratin, type I cytoskeletal 12 (KRT12) ELISA Kit employs a two-site sandwich ELISA to quantitate KRT12 in samples. An antibody specific for KRT12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KRT12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Keratin 12 is a keratin found expressed in corneal epithelia. Mutations in the gene encoding this protein lead to Meesmann corneal dystrophy. To elucidate the function of keratin 12, Kao et al. (1996) created knockout mice lacking the Krt1.12 gene by gene targeting techniques. The heterozygous mice appeared normal. Homozygous mice developed normally and suffered mild corneal epithelial erosion. The corneal epithelia were fragile and could be removed by gentle rubbing of the eyes or brushing. The corneal epithelium of the homozygotes did not express keratin 12 as judged by Immunohistochemistry, Western immunoblot analysis with epitope-specific anti-keratin 12 antibodies, Northern hybridization, and in situ hybridization with an antisense keratin 12 riboprobe.

NCBI and Uniprot Product Information

• NCBI GI #: 4557699
• NCBI GeneID: 3859
• NCBI Accession #: NP_000214.1
• NCBI GenBank Nucleotide #: NP_000214.1
• UniProt Accession #: Q99456
• NCBI Official Full Name: keratin, type I cytoskeletal 12
• NCBI Official Synonym Full Names: keratin 12
• NCBI Official Symbol: KRT12
• NCBI Official Synonym Symbols: K12
• NCBI Protein Information: keratin, type I cytoskeletal 12
• UniProt Protein Name: Keratin, type I cytoskeletal 12
• Protein Family: Keratin
• UniProt Gene Name: KRT12
• UniProt Synonym Gene Names: CK-12; K12
• UniProt Entry Name: K1C12_HUMAN

NCBI Description

KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity

By similarity.

Subunit structure: Heterotetramer of two type I and two type II keratins. Keratin-3 associates with keratin-12.

Tissue specificity: Cornea specific. Ref.1

Involvement in disease: Corneal dystrophy, Meesmann (MECD) [MIM:122100]: An autosomal dominant corneal disease characterized by fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.2 Ref.3 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13

Miscellaneous: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Sequence similarities: Belongs to the intermediate filament family.