Human Keratin 1 (KRT1) ELISA Kit | KRT1 elisa kit

Human Keratin 1 (KRT1) ELISA Kit

Cat. Num. AAA2021265

• Gene Names: KRT1; K1; CK1; EHK; EHK1; EPPK; KRT1A; NEPPK
• Reactivity: Human
• Synonyms: Keratin 1 (KRT1); Human Keratin 1 (KRT1) ELISA Kit; CK1; EHK1; K1; KRT1A; KRT1-A; Cytokeratin 1; Epidermolytic Hyperkeratosis; Keratin; type II cytoskeletal 1; 67 kDa cytokeratin; Type-II keratin Kb1; KRT1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of KRT1. No significant cross-reactivity or interference between KRT1 and analogues was observed.
• Sequence Length: 644

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level KRT1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level KRT1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KRT1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of KRT1 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to KRT1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to KRT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain KRT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of KRT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for KRT1 elisa kit

Tumor immunity; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 186772
• NCBI GeneID: 3848
• UniProt Accession #: P04264; P85925; P86104; Q14720; Q6GSJ0; Q9H298; B2RA01
• Molecular Weight: 66,039 Da
• NCBI Official Full Name: keratin 1
• NCBI Official Synonym Full Names: keratin 1, type II
• NCBI Official Symbol: KRT1
• NCBI Official Synonym Symbols: K1; CK1; EHK; EHK1; EPPK; KRT1A; NEPPK
• NCBI Protein Information: keratin, type II cytoskeletal 1
• UniProt Protein Name: Keratin, type II cytoskeletal 1
• Protein Family: Keratin
• UniProt Gene Name: KRT1
• UniProt Synonym Gene Names: KRTA; CK-1; K1
• UniProt Entry Name: K2C1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]

Uniprot Description

K1: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K10.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: extracellular matrix; extracellular space; cytoskeleton; membrane; keratin filament; plasma membrane; nucleus

Molecular Function: protein binding; receptor activity; structural molecule activity; carbohydrate binding

Biological Process: retinal homeostasis; fibrinolysis; negative regulation of inflammatory response; response to oxidative stress; complement activation, lectin pathway; regulation of angiogenesis

Disease: Palmoplantar Keratoderma, Epidermolytic; Keratosis Palmoplantaris Striata Iii; Ichthyosis Hystrix, Curth-macklin Type; Palmoplantar Keratoderma, Nonepidermolytic; Epidermolytic Hyperkeratosis; Ichthyosis, Cyclic, With Epidermolytic Hyperkeratosis