Western Blot (WB)
(Western blot analysis of GPR124 expression in CT26 (A), PMVEC (B), MEF (C), A549 (D), LOVO (E) whole cell lysates.)
Rabbit GPR124 Polyclonal Antibody | anti-GPR124 antibody
Anti-GPR124 Antibody
Gene Names
ADGRA2; TEM5; GPR124
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Purified by immunogen affinity chromatography.
Synonyms
GPR124; Polyclonal Antibody; Anti-GPR124 Antibody; KIAA1531; TEM5; G-protein coupled receptor 124; Tumor endothelial marker 5; anti-GPR124 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of GPR124 protein.
Purity/Purification
Purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Sequence Length
1338
Applicable Applications for anti-GPR124 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GPR124.
Preparation and Storage
Upon delivery aliquot and store at-20 degree C for one year. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of GPR124 expression in CT26 (A), PMVEC (B), MEF (C), A549 (D), LOVO (E) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of GPR124 expression in CT26 (A), PMVEC (B), MEF (C), A549 (D), LOVO (E) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of GPR124 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of GPR124 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Related Product Information for anti-GPR124 antibody
Rabbit polyclonal antibody to GPR124
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
119,862 Da
NCBI Official Full Name
adhesion G protein-coupled receptor A2
NCBI Official Synonym Full Names
adhesion G protein-coupled receptor A2
NCBI Official Symbol
ADGRA2
NCBI Official Synonym Symbols
TEM5; GPR124
NCBI Protein Information
adhesion G protein-coupled receptor A2
UniProt Protein Name
Adhesion G protein-coupled receptor A2
UniProt Gene Name
ADGRA2
UniProt Synonym Gene Names
GPR124; KIAA1531ImportedManual assertion inferred from database entriesiHGNC:17849; TEM51 PublicationManual assertion based on opinion iniRef.1
Uniprot Description
Endothelial receptor which functions as a WNT7-specific coactivator of canonical Wnt signaling (). Required for normal endothelial cell sprouting and migration in the forebrain and neural tube (). Has a major role in establishing the blood-brain barrier (). Binds to the glycosaminoglycans heparin, heparin sulfate, chondroitin sulfate and dermatan sulfate (PubMed:16982628).
Research Articles on GPR124
Similar Products
Product Notes
The GPR124 adgra2 (Catalog #AAA8304138) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GPR124 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GPR124 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the GPR124 adgra2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPR124, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
