Human Kallikrein 1 (KLK1) ELISA Kit | KLK1 elisa kit
Human Kallikrein 1 (KLK1) ELISA Kit
Gene Names
KLK1; hK1; KLKR; Klk6
Reactivity
Human
Synonyms
Kallikrein 1 (KLK1); Human Kallikrein 1 (KLK1) ELISA Kit; KLK1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of KLK1. No significant cross-reactivity or interference between KLK1 and analogues was observed.
Sequence Length
262
Samples
Human serum, plasma and other biological fluids
Detection Range
18.8-1,200pg/mL
Sensitivity
The minimum detectable dose of KLK1 is typically less than 7.4pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level KLK1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level KLK1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Intended Uses
The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of KLK1 in human serum, plasma and other biological fluids.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Related Product Information for KLK1 elisa kit
Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to KLK1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to KLK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain KLK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of KLK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,890 Da
NCBI Official Full Name
kallikrein-1 preproprotein
NCBI Official Synonym Full Names
kallikrein 1
NCBI Official Symbol
KLK1
NCBI Official Synonym Symbols
hK1; KLKR; Klk6
NCBI Protein Information
kallikrein-1; tissue kallikrein; glandular kallikrein 1; kallikrein serine protease 1; kallikrein 1, renal/pancreas/salivary; kidney/pancreas/salivary gland kallikrein
UniProt Protein Name
Kallikrein-1
Protein Family
UniProt Gene Name
KLK1
UniProt Entry Name
KLK1_HUMAN
NCBI Description
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen. [provided by RefSeq, Jul 2008]
Uniprot Description
KLK1: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Belongs to the peptidase S1 family. Kallikrein subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease; EC 3.4.21.35
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: nucleus
Molecular Function: serine-type endopeptidase activity
Biological Process: proteolysis
Disease: Kallikrein, Decreased Urinary Activity Of
Research Articles on KLK1
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Product Notes
The Human KLK1 klk1 (Catalog #AAA2023375) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2023375 ELISA Kit recognizes Human KLK1. It is sometimes possible for the material contained within the vial of "Kallikrein 1 (KLK1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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